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. 2024 Jul;119(2):1059-1072.
doi: 10.1111/tpj.16816. Epub 2024 May 18.

Restricted responses of AcMYB68 and AcERF74/75 enhanced waterlogging tolerance in kiwifruit

Yue Chen #  1 , Wen-Yue Su #  1 , Chun-Jiang Ren  1 , Yi-Lai Lin  1 , Wen-Qiu Wang  1 , Hui-Qin Zhang  2 , Xue-Ren Yin  1   3 , Xiao-Fen Liu  1   3
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Free article

Restricted responses of AcMYB68 and AcERF74/75 enhanced waterlogging tolerance in kiwifruit

Yue Chen et al. Plant J. 2024 Jul.
Free article

Abstract

Most of kiwifruit cultivars (e.g. Actinidia chinensis cv. Donghong, "DH") were sensitive to waterlogging, thus, waterlogging resistant rootstocks (e.g. Actinidia valvata Dunn, "Dunn") were widely used for kiwifruit industry. Those different species provided ideal materials to understand the waterlogging responses in kiwifruit. Compared to the weaken growth and root activities in "DH", "Dunn" maintained the relative high root activities under the prolonged waterlogging. Based on comparative analysis, transcript levels of pyruvate decarboxylase (PDCs) and alcohol dehydrogenase (ADHs) showed significantly difference between these two species. Both PDCs and ADHs had been significantly increased by waterlogging in "DH", while they were only limitedly triggered by 2 days stress and subsided during the prolonged waterlogging in "Dunn". Thus, 19 differentially expressed transcript factors (DETFs) had been isolated using weighted gene co-expression network analysis combined with transcriptomics and transcript levels of PDCs and ADHs in waterlogged "DH". Among these DETFs, dual luciferase and electrophoretic mobility shift assays indicated AcMYB68 could bind to and trigger the activity of AcPDC2 promoter. The stable over-expression of AcMYB68 significantly up-regulated the transcript levels of PDCs but inhibited the plant growth, especially the roots. Moreover, the enzyme activities of PDC in 35S::AcMYB68 were significantly enhanced during the waterlogging response than that in wild type plants. Most interestingly, comparative analysis indicated that the expression patterns of AcMYB68 and the previously characterized AcERF74/75 (the direct regulator on ADHs) either showed no responses (AcMYB68 and AcERF74) or very limited response (AcERF75) in "Dunn". Taken together, the restricted responses of AcMYB68 and AcERF74/75 in "Dunn" endow its waterlogging tolerance.

Keywords: Actinidia chinensis; Actinidia valvata; MYB; root activities; waterlogging stress.

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References

REFERENCES

    1. Armstrong, W. (1980) Aeration in higher plants. Advances in Botanical Research, 7, 225–332.
    1. Bai, D.F., Li, Z., Qi, X.J., Chen, J.Y., Gu, H., Huang, W.Q. et al. (2019) Physiological responses and tolerance evaluation of four species of Actinidia to waterlogging stress. Journal of Fruit Science, 36, 163–173.
    1. Borrego‐Benjumea, A., Carter, A., Tucker, J.R., Yao, Z., Xu, W. & Badea, A. (2020) Genome‐wide analysis of gene expression provides new insights into waterlogging responses in barley (Hordeum vulgare L.). Plants, 9(2), 240.
    1. Gao, J.F. (2006) Plant physiology experiment guidance. Beijing: Higher Education Press.
    1. Gao, Y., Shi, M., Zhou, Y., Huo, L., Li, X. & Xu, K. (2022) Comparative transcriptome provides insight into responding mechanism of waterlogging stress in Actinidia valvata Dunn. Gene, 845, 146843.

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