Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 18;206(6):267.
doi: 10.1007/s00203-024-03992-8.

Exploring the evolutionary and pathogenic role of Acinetobacter baumannii biofilm-associated protein (Bap) through in silico structural modeling

Kirti Upmanyu  1   2 , Rakesh Kumar  1 , Qazi Mohd Rizwanul Haque  2 , Ruchi Singh  3
Affiliations

Exploring the evolutionary and pathogenic role of Acinetobacter baumannii biofilm-associated protein (Bap) through in silico structural modeling

Kirti Upmanyu et al. Arch Microbiol. .

Abstract

Acinetobacter species encode for extracellularly secreted Biofilm-associated protein (Bap), a multi-domain protein with variable molecular weights reaching several hundred kilodaltons. Bap is crucial for the development of multi-dimensional structures of mature biofilms. In our investigation, we analyzed 7338 sequences of A. baumannii from the NCBI database and found that Bap or Bap-like protein (BLP) was present in 6422 (87.52%) isolates. Further classification revealed that 12.12% carried Type-1 Bap, 68.44% had Type-2, 6.91% had Type-3, 0.05% had Type-6 or SDF-Type, and 12.51% lacked Bap or BLP. The majority of isolates with Type-1, Type-2, and Type-3 Bap belonged to ST1, ST2, and ST25, respectively. Phylogenetic analysis suggested that Type-1 Bap is the most ancient, while Type-3 and SDF-Type have evolved recently. Studying the interaction of predicted Bap structures with human CEACAM-1 and PIgR showed that Bap with its BIg13 and BIg6 domains interact with the N-terminal domain of CEACAM-1, involving Arg43 and Glu40, involved in CEACAM-1 dimerization. Also, we found that recently evolved Type-3 and SDF-Type Bap showed greater interaction with CEACAM-1 and PIgR. It can be asserted that the evolution of Bap has conferred enhanced virulence characteristics to A. baumannii with increased interaction with CEACAM-1 and PIgR. Using in silico approaches, this study explores the evolutionary, physicochemical, and structural features of A. baumannii Bap and unravels its crucial role in mediating interaction with human CEACAM-1 and PIgR through detailed structure modelling. These findings advance our understanding of A. baumannii Bap and highlight its role in pathogenesis.

Keywords: A. Baumannii; Biofilm; Biofilm associated protein (Bap); CEACAM-1; Host pathogen interaction; PIgR; Pathogenesis; Virulence.

PubMed Disclaimer

References

    1. Agarwal V, Asmat TM, Dierdorf NI, Hauck CR, Hammerschmidt S (2010) Polymeric immunoglobulin receptor-mediated invasion of Streptococcus pneumoniae into host cells requires a coordinate signaling of SRC family of protein-tyrosine kinases, ERK, and c-Jun N-terminal kinase. J Biol Chem 285(46):35615–35623. https://doi.org/10.1074/jbc.M110.172999 - DOI - PubMed - PMC
    1. Alsan M, Klompas M (2010) Acinetobacter baumannii: an emerging and important pathogen. J Clin Outcomes Management: JCOM 17(8):363
    1. Ambrosi C, Scribano D, Sarshar M, Zagaglia C, Singer BB, Palamara AT (2020) Acinetobacter baumannii targets human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) for invasion of pneumocytes. Msystems 5(6):10–1128. https://doi.org/10.1128/msystems.00604-20 - DOI
    1. Bamberger AM, Sudahl S, Löning T, Wagener C, Bamberger CM, Drakakis P, Coutifaris C, Makrigiannakis A (2000) The adhesion molecule CEACAM1 (CD66a, C-CAM, BGP) is specifically expressed by the extravillous intermediate trophoblast. Am J Pathol 156(4):1165–1170. https://doi.org/10.1016/S0002-9440(10)64985-1 - DOI - PubMed - PMC
    1. Baranov V, Hammarström S (2004) Carcinoembryonic antigen (CEA) and CEA-related cell adhesion molecule 1 (CEACAM1), apically expressed on human colonic M cells, are potential receptors for microbial adhesion. Histochem Cell Biol 121:83–89. https://doi.org/10.1007/s00418-003-0613-5 - DOI - PubMed

MeSH terms

LinkOut - more resources