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. 2024 May;44(3):551-569.
doi: 10.5851/kosfa.2024.e64. Epub 2024 May 1.

Study on the Digestion-Induced Changes in the Characteristics and Bioactivity of Korean Native and Overseas Cattle-Derived Peptides

Jae Hyeon Kim  1 , Da Young Lee  1 , Seung Yun Lee  2 , Ermie Mariano Jr  1 , Jae Won Jeong  1 , Seung Hyeon Yun  1 , Juhyun Lee  1 , Jinmo Park  1 , Yeongwoo Choi  1 , Dahee Han  1 , Jin Soo Kim  1 , Cheorun Jo  3 , Sun Jin Hur  1
Affiliations

Study on the Digestion-Induced Changes in the Characteristics and Bioactivity of Korean Native and Overseas Cattle-Derived Peptides

Jae Hyeon Kim et al. Food Sci Anim Resour. 2024 May.

Abstract

This study was conducted to compare and analyze the changes in the biochemical characteristics and biological activity of peptide extracts derived from Chickso, Hanwoo, and Wagyu beef during digestion. The results of the in vitro digestion analysis revealed that the digestion rate, total free amino acid content, and antioxidant and antihypertensive activities of Chickso loin and shank myofibrillar proteins were significantly higher (p<0.05) than those of Hanwoo and Wagyu loin and shank myofibrillar proteins. Particularly, the peptide extracts of Chickso loin and shank had a high angiotensin-converting enzyme inhibitory activity. In mice in vivo digestion experiment, the blood serum of mice fed with Chickso loin peptide extract (<10 kDa) showed the highest antioxidant enzyme activity. Thus, Chickso peptide extracts were deemed to be similar or more bioactive than Hanwoo and Wagyu peptide extracts, and can be used as bioactive materials.

Keywords: Chickso; Korean native cattle; antihypertensive activity; antioxidant activity; peptide extracts.

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Conflict of interest statement

The authors declare no potential conflicts of interest.

Figures

Fig. 1.
Fig. 1.. The process of preparing myofibrillar proteins and peptide extracts (<10 kDa) from Chickso, Hanwoo, and Wagyu beef.
DW, distilled water; PBS, phosphate buffer.
Fig. 2.
Fig. 2.. Changes in the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts due to in vitro digestion.
A comparison of the ABTS radical scavenging activity of the peptides before and after digestion. Data are presented as mean±SD. * p<0.05 and ** p<0.001 indicate a significant difference according to digestion.
Fig. 3.
Fig. 3.. Changes in the 2,2-diphenyl-1-picrylhudrazyl (DPPH) radical scavenging activity of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts due to in vitro digestion.
A comparison of the DPPH radical scavenging activity of the peptides before and after digestion. Data are presented as mean±SD. ** p<0.001 indicates a significant difference according to digestion.
Fig. 4.
Fig. 4.. Changes in the iron chelating activity of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts due to in vitro digestion.
A comparison of the iron chelating activity of the peptides before and after digestion. * p<0.05 indicates a significant difference according to digestion.
Fig. 5.
Fig. 5.. Changes in the reducing power of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts due to in vitro digestion.
A comparison of the reducing power of the peptides before and after digestion. * p<0.05 indicates a significant difference according to digestion.
Fig. 6.
Fig. 6.. Changes in the angiotensin-converting enzyme (ACE) inhibitory activity of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts due to in vitro digestion.
A comparison of the ACE inhibitory activity of the peptides before and after digestion. Data are presented as mean±SD. ** p<0.001 indicates a significant difference according to digestion.
Fig. 7.
Fig. 7.. The antioxidant enzyme activities of mice plasma which fed with the peptide extracts derived from the loin and shank of Chickso, Hanwoo, and Wagyu.
(A) Catalase activity, (B) peroxidase activity, (C) superoxide dismutase (SOD) activity. Data are presented as mean±SD. A–F Different uppercase letters indicate a significant difference based on breeds and cuts (p<0.05). CTL, Control.
Fig. 8.
Fig. 8.. The angiotensin-converting enzyme (ACE) activity of Chickso, Hanwoo, and Wagyu loin and shank peptide extracts (<10 kDa) after in vivo digestion in mice.
Data are presented as mean±SD. CTL, Control.
See this image and copyright information in PMC

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