doi: 10.1128/jb.140.2.734-737.1979.
Selection for Escherichia coli mutants with proteins missing from the ribosome
- PMID: 387748
- PMCID: PMC216704
- DOI: 10.1128/jb.140.2.734-737.1979
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Selection for Escherichia coli mutants with proteins missing from the ribosome
J Bacteriol.
1979 Nov.
Abstract
Antibiotic-independent revertants of an erythromycin-dependent strain of Escherichia coli were isolated by spontaneous selection. Their ribosomal proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. In contrast to most ribosomally targeted selections, the specific absence of a certain protein from the ribosome, rather than alterations in ribosomal proteins, was observed. Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing.
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