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. 2024 May 22;19(5):e0303567.
doi: 10.1371/journal.pone.0303567. eCollection 2024.

Recent advances in cryotolerance biomarkers for semen preservation in frozen form-A systematic review

Jiajia Suo  1   2 , Jieru Wang  1 , Yanling Zheng  2 , Fayi Xiao  2 , Ruchun Li  2 , Fei Huang  1 , Peng Niu  1 , Wei Zhu  2 , Xiaoxia Du  2 , Jianxiu He  2 , Qinghua Gao  1 , Ahrar Khan  2   3
Affiliations

Recent advances in cryotolerance biomarkers for semen preservation in frozen form-A systematic review

Jiajia Suo et al. PLoS One. .

Abstract

Spermatozoa cryopreservation has been practiced for decades and is a very useful technique for long-term preservation of sperm fertility. The capability for semen cryopreservation varies across species, seasons, latitudes, and even for different ejaculates from the same animal. This article summarizes research results on sperm cryotolerance biomarkers in several species, focusing on three areas: spermatozoa cryotolerance biomarkers, seminal plasma proteins cryotolerance biomarkers, and other cryotolerance biomarkers. We discovered that sperm cryoresistance biomarkers are primarily related to sperm plasma membrane stability, the presence of antioxidant substances in sperm or seminal plasma, sperm cell energy metabolism, water and small molecule transport channels in the sperm plasma membrane, and antistress substances in sperm or seminal plasma. The research conducted using diverse livestock models can be employed to enhance the basic and applied reproduction of other mammals through the study of sperm cryotolerance biomarkers, as well as the substantial similarities between livestock and other organisms, including endangered species.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. PRISMA 2020 Flow diagram of the study selection for systematic review.
Studies reporting association: 15 studies in this review reported 18 biomarkers of spermatozoa, 9 studies in this review reported 13 biomarkers of seminal plasma, 4 studies in this review reported 6 other cryotolerance biomarkers.
Fig 2
Fig 2. Types of cryotolerance biomarkers of spermatozoa.
See this image and copyright information in PMC

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