Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Oct;47(10):3766-3779.
doi: 10.1111/pce.14958. Epub 2024 May 23.

The interplay of growth-regulating factor 5 and BZR1 in coregulating chlorophyll degradation in poplar

Hao Chen  1   2 , Wenqi Wu  3 , Kang Du  1   2 , Aoyu Ling  1   2 , Xiangyang Kang  1   2
Affiliations

The interplay of growth-regulating factor 5 and BZR1 in coregulating chlorophyll degradation in poplar

Hao Chen et al. Plant Cell Environ. 2024 Oct.

Abstract

Chlorophyll (Chl) is essential for plants to carry out photosynthesis, growth and development processes. Growth-regulating factors (GRFs) play a vital role in regulating Chl degradation in plants. However, the molecular mechanism by which GRF5 regulates Chl degradation in poplar remains unknown. Here we found that overexpression of PpnGRF5-1 increased Chl content in leaves and promoted chloroplast development in poplar. Overexpression of PpnGRF5-1 in poplar delayed Chl degradation induced by external factors, such as hormones, darkness and salt stress. PpnGRF5-1 responded to brassinosteroid (BR) signalling during BR-induced Chl degradation and reduced the expression levels of Chl degradation and senescence-related genes. PpnGRF5-1 inhibited the expression of Chl b reductases PagNYC1 and PagNOL. PpnGRF5-1 could interact with PagBZR1 in the nucleus. PagBZR1 also inhibited the expression of PagNYC1. In addition, we found that the protein-protein interaction between PagBZR1 and PpnGRF5-1 enhanced the inhibitory effect of PpnGRF5-1 on the Chl b reductases PagNYC1 and PagNOL. BZR1 and GRF5-1 were upregulated, and NOL and NYC1 were downregulated in triploid poplars compared to diploids. This study revealed a new mechanism by which PpnGRF5-1 regulates Chl degradation in poplars and lays the foundation for comprehensively analysing the molecular mechanism of Chl metabolism in triploid poplars.

Keywords: PpnGRF5‐1; brassinosteroid.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

REFERENCES

    1. Bailey, P.C., Martin, C., Toledo‐Ortiz, G., Quail, P.H., Huq, E., Heim, M.A. et al. (2003) Update on the basic helix‐loop‐helix transcription factor gene family in Arabidopsis thaliana. The Plant Cell, 15, 2497–2502.
    1. Belkhadir, Y. & Jaillais, Y. (2015) The molecular circuitry of brassinosteroid signaling. New Phytologist, 206, 522–540.
    1. Chen, H., Li, J., Qiu, B., Zhao, Y., Liu, Z. & Yang, J. et al. (2021) Long non‐coding RNA and its regulatory network response to cold stress in Eucalyptus urophylla S. T. Blake. Forests, 12(7), 836.
    1. Chen, Q., Yan, J., Tong, T., Zhao, P., Wang, S., Zhou, N. et al. (2023a) ANAC087 transcription factor positively regulates age‐dependent leaf senescence through modulating the expression of multiple target genes in Arabidopsis. Journal of Integrative Plant Biology, 65, 967–984.
    1. Chen, W., Zheng, Y.C., Wang, J.Y., Wang, Z.J., Yang, Z., Chi, X.Y. et al. (2023b) Ethylene‐responsive SbWRKY50 suppresses leaf senescence by inhibition of chlorophyll degradation in sorghum. New Phytologist, 238, 1129–1145.

MeSH terms

LinkOut - more resources