Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1985 Dec;82(24):8404-8.
doi: 10.1073/pnas.82.24.8404.

Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector

G E Smith, G Ju, B L Ericson, J Moschera, H W Lahm, R Chizzonite, M D Summers
Comparative Study

Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector

G E Smith et al. Proc Natl Acad Sci U S A. 1985 Dec.

Abstract

A cDNA coding for human interleukin 2 (IL-2) was inserted into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus produced high levels of Mr 15,500 IL-2 polypeptide, the majority of which was secreted into the culture medium during infection. The recombinant IL-2 was able to stimulate the growth of an IL-2-dependent cell line. The N-terminal amino acid sequence of the insect-derived IL-2 was identical to that of natural IL-2. Thus, a mammalian signal peptide was recognized and properly removed in insect cells.

PubMed Disclaimer

References

    1. Mol Immunol. 1981 Dec;18(12):1087-94 - PubMed
    1. Nature. 1970 May 2;226(5244):466-7 - PubMed
    1. J Immunol. 1978 Jun;120(6):2027-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Oct;81(20):6486-90 - PubMed
    1. J Virol. 1980 Mar;33(3):1111-21 - PubMed

Publication types

LinkOut - more resources