Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 15;10(5):352.
doi: 10.3390/jof10050352.

Genome Characteristics of the Endophytic Fungus Talaromyces sp. DC2 Isolated from Catharanthus roseus (L.) G. Don

Nguyen Duc Quan  1 , Ngoc-Lan Nguyen  1   2 , Tran Thi Huong Giang  1 , Nguyen Thi Thanh Ngan  1   2 , Nguyen Thanh Hien  1 , Nguyen Van Tung  1   2 , Nguyen Hoang Thanh Trang  1 , Nguyen Thi Kim Lien  1   2 , Huy Hoang Nguyen  1   2
Affiliations

Genome Characteristics of the Endophytic Fungus Talaromyces sp. DC2 Isolated from Catharanthus roseus (L.) G. Don

Nguyen Duc Quan et al. J Fungi (Basel). .

Abstract

Talaromyces sp. DC2 is an endophytic fungus that was isolated from the stem of Catharanthus roseus (L.) G. Don in Hanoi, Vietnam and is capable of producing vinca alkaloids. This study utilizes the PacBio Sequel technology to completely sequence the whole genome of Talaromyces sp. DC2The genome study revealed that DC2 contains a total of 34.58 Mb spanned by 156 contigs, with a GC content of 46.5%. The identification and prediction of functional protein-coding genes, tRNA, and rRNA were comprehensively predicted and highly annotated using various BLAST databases, including non-redundant (Nr) protein sequence, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Carbohydrate-Active Enzymes (CAZy) databases. The genome of DC2 has a total of 149, 227, 65, 153, 53, and 6 genes responsible for cellulose, hemicellulose, lignin, pectin, chitin, starch, and inulin degradation, respectively. The Antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analyses revealed that strain DC2 possesses 20 biosynthetic gene clusters responsible for producing secondary metabolites. The strain DC2 has also been found to harbor the DDC gene encoding aromatic L-amino acid decarboxylase enzyme. Conclusively, this study has provided a comprehensive understanding of the processes involved in secondary metabolites and the ability of the Talaromyces sp. DC2 strain to degrade plant cell walls.

Keywords: Catharanthus roseus; DC2; DDC gene; Talaromyces; endophytic fungus; genome sequencing; plant cell wall degradation; secondary metabolites.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Kyoto Encyclopedia of Genes and Genomes (KEGG) functional classification of Talaromyces sp. DC2.
Figure 2
Figure 2
Distribution of carbohydrate-active enzyme (CAZyme) familes (A), and signal and transmembrane CAZymes (B) in Talaromyces sp. DC2.
Figure 3
Figure 3
Comparison of biosynthetic gene cluster constituents in strain DC2 with identified biosynthetic gene clusters for biosynthesis of swainsonine (66%), varicidin A, asperterpenoid A, squalestatin S1, ustethylin A, trichobrasilenol/xylarenic acid B/brasilane A/F/E/D, and ilicicolin H.
Figure 4
Figure 4
The indole alkaloid biosynthetic pathway gene found in Talaromyces sp. DC2 is depicted by the red-colored box. AADC, aromatic L-amino acid decarboxylase.
Figure 5
Figure 5
Phylogenetic tree includes the amino acid sequences of putative aromatic L-amino acid decarboxylases. MUSCLE v.5.0 was used for sequence alignment and a neighbor-joining algorithm was used to construct the tree in the Mega-X v.10.2.6.
See this image and copyright information in PMC

References

    1. Das A., Sarkar S., Bhattacharyya S., Gantait S. Biotechnological Advancements in Catharanthus roseus (L.) G. Don. Appl. Microbiol. Biotechnol. 2020;104:4811–4835. doi: 10.1007/s00253-020-10592-1. - DOI - PubMed
    1. Mujib A., Ilah A., Aslam J., Fatima S., Siddiqui Z.H., Maqsood M. Catharanthus roseus Alkaloids: Application of Biotechnology for Improving Yield. Plant Growth Regul. 2012;68:111–127. doi: 10.1007/s10725-012-9704-4. - DOI
    1. Kumar S., Singh B., Singh R. Catharanthus roseus (L.) G. Don: A Review of Its Ethnobotany, Phytochemistry, Ethnopharmacology and Toxicities. J. Ethnopharmacol. 2022;284:114647. doi: 10.1016/j.jep.2021.114647. - DOI - PubMed
    1. Kumar A., Patil D., Rajamohanan P.R., Ahmad A. Isolation, Purification and Characterization of Vinblastine and Vincristine from Endophytic Fungus Fusarium oxysporum Isolated from Catharanthus roseus. PLoS ONE. 2013;8:e71805. doi: 10.1371/journal.pone.0071805. - DOI - PMC - PubMed
    1. Palem P.P.C., Kuriakose G.C., Jayabaskaran C. An Endophytic Fungus, Talaromyces radicus, Isolated from Catharanthus roseus, Produces Vincristine and Vinblastine, Which Induce Apoptotic Cell Death. PLoS ONE. 2015;10:e0144476. doi: 10.1371/journal.pone.0144476. - DOI - PMC - PubMed