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. 2024 May 9;11(5):208.
doi: 10.3390/vetsci11050208.

Establishment of a Multilocus Sequence Typing Scheme for the Characterization of Avibacterium paragallinarum

Mengjiao Guo  1 , Yikun Jin  1 , Haonan Wang  1 , Xiaorong Zhang  1 , Yantao Wu  1   2
Affiliations

Establishment of a Multilocus Sequence Typing Scheme for the Characterization of Avibacterium paragallinarum

Mengjiao Guo et al. Vet Sci. .

Abstract

Infectious coryza is an acute respiratory infection caused by Avibacterium paragallinarum, which is widely distributed throughout the world. However, there is no effective molecular typing scheme to obtain basic knowledge about the Av. paragallinarum population structure. This study aimed to develop a multilocus sequence typing (MLST) scheme for Av. paragallinarum that allows for the worldwide comparison of sequence data. For this purpose, the genetic variability of 59 Av. paragallinarum strains from different geographical origins and serovars was analyzed to identify correlations. The MLST scheme was developed using seven conserved housekeeping genes, which identified eight STs that clustered all of the strains into three evolutionary branches. The analytical evaluation of the clone group relationship between the STs revealed two clone complexes (CC1 and CC2) and three singletons (ST2, ST5, and ST6). Most of the isolates from China belonged to ST1 and ST3 in CC1. ST8 from Peru and ST7 from North America together formed CC2. Our results showed that the Av. paragallinarum strains isolated from China had a distant genetic relationship with CC2, indicating strong regional specificity. The MLST scheme established in this study can monitor the dynamics and genetic differences of Av. paragallinarum transmission.

Keywords: Avibacterium paragallinarum; housekeeping gene; multilocus sequence typing; polymorphism.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Phylogenetic tree showing the relatedness of 59 Av. paragallinarum strains generated from MLST sequences.
Figure 2
Figure 2
Cluster analysis for STs of 59 Av. paragallinarum strains.
See this image and copyright information in PMC

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References

    1. Blackall P.J. Infectious coryza: Overview of the disease and new diagnostic options. Clin. Microbiol. Rev. 1999;12:627–632. doi: 10.1128/CMR.12.4.627. - DOI - PMC - PubMed
    1. Blackall P.J., Soriano-Vargas E. Infectious coryza and related bacterial infections. Dis. Poult. 2020:890–906. doi: 10.1002/9781119371199.ch20. - DOI
    1. Blackall P.J., Morrow C.J., McInnes A., Eaves L.E., Rogers D.G. Epidemiologic studies on infectious coryza outbreaks in northern New South Wales, Australia, using serotyping, biotyping, and chromosomal DNA restriction endonuclease analysis. Avian Dis. 1990;34:267–276. doi: 10.2307/1591408. - DOI - PubMed
    1. Page L.A. Haemophilus infections in chickens. I. Characteristics of 12 Haemophilus isolates recovered from diseased chickens. Am. J. Vet. Res. 1962;23:85–95. - PubMed
    1. Kume K., Sawata A., Nakai T., Matsumoto M. Serological classification of Haemophilus paragallinarum with a hemagglutinin system. J. Clin. Microbiol. 1983;17:958–964. doi: 10.1128/jcm.17.6.958-964.1983. - DOI - PMC - PubMed

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