Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 16;29(10):2348.
doi: 10.3390/molecules29102348.

Structure Identification of Ganoderma lucidum Spore Polysaccharides and Their Antitumor Activity In Vivo

Hui-Min Liu  1   2 , Jun Cheng  1 , Xiao-Yi Wang  1 , Yan Jiang  3 , Jia Ni  1 , Yun Zhang  1 , Wei Wang  1   2
Affiliations

Structure Identification of Ganoderma lucidum Spore Polysaccharides and Their Antitumor Activity In Vivo

Hui-Min Liu et al. Molecules. .

Abstract

Ganoderma lucidum spore powder, valued for its nutritional and medicinal properties, contains polysaccharides crucial for its efficacy. However, the complex structural nature of these polysaccharides necessitates further investigation to fully realize their potential. This study aimed to investigate the effects of acid heat treatment on Ganoderma lucidum spore polysaccharides (GLSPs) to enhance their properties and application in antitumor activity. The GLSP was obtained via acid heat treatment, concentration, and centrifugal separation. This process led to a notable reduction in polysaccharide molecular weight, increasing water solubility and bioavailability. Analytical techniques including NMR spectroscopy and methylation analysis revealed a polysaccharide composition comprising four distinct monosaccharides, with molecular weights of 3291 Da (Mw) and 3216 Da (Mn). Six different linkage modes were identified, with a molar ratio of 1:5:2:3:4:3. In vivo experiments demonstrated the GLSP's significant inhibitory effect on the growth of four tumor models (sarcoma S180, Lewis lung cancer, liver cancer H22, and colon cancer C26) in mice, with no observed toxicity. These findings suggest the GLSP's potential as an antitumor therapeutic agent for clinical use.

Keywords: Ganoderma lucidum spore polysaccharide; antitumor activity; efficacy; structural nature.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
High-performance gel permeation chromatogram of GLSP.
Figure 2
Figure 2
The FTIR spectrum of the GLSP.
Figure 3
Figure 3
(A) Ion chromatogram of 16 monosaccharides of mixed standards. (B) Ion chromatogram of GLSPs.
Figure 3
Figure 3
(A) Ion chromatogram of 16 monosaccharides of mixed standards. (B) Ion chromatogram of GLSPs.
Figure 4
Figure 4
1D-NMR spectra of GLSP. (A) 1H spectrum; (B) 13C spectrum.
Figure 5
Figure 5
2D-NMR spectra of GLSP. (A) 1H–1H COSY spectrum; (B) HSQC spectrum; (C) HMBC spectrum.
Figure 5
Figure 5
2D-NMR spectra of GLSP. (A) 1H–1H COSY spectrum; (B) HSQC spectrum; (C) HMBC spectrum.
Figure 6
Figure 6
Structure of GLSP.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cao W., Chen H.-D., Yu Y.-W., Li N., Chen W.-Q., Ni J. Changing profiles of cancer burden worldwide and in China: A secondary analysis of the global cancer statistics 2020. Chin. Med. J. 2021;134:783–791. doi: 10.1097/CM9.0000000000001474. - DOI - PMC - PubMed
    1. Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA A Cancer J. Clin. 2018;68:394–424. doi: 10.3322/caac.21492. - DOI - PubMed
    1. Ribelles N., Pascual J., Galvez-Carvajal L., Ruiz-Medina S., Garcia-Corbacho J., Benitez J.C., Dominguez-Recio M.E., Torres E., Oliva L., Zalabardo M., et al. Increasing Annual Cancer Incidence in Patients Age 20–49 Years: A Real-Data Study. JCO Glob. Oncol. 2024;10:e2300363. doi: 10.1200/GO.23.00363. - DOI - PMC - PubMed
    1. Liu Y.-P., Zheng C.-C., Huang Y.-N., He M.-L., Xu W.W., Li B. Molecular mechanisms of chemo- and radiotherapy resistance and the potential implications for cancer treatment. MedComm. 2021;2:315–340. doi: 10.1002/mco2.55. - DOI - PMC - PubMed
    1. Tangkiatkumjai M., Boardman H., Walker D.-M. Potential factors that influence usage of complementary and alternative medicine worldwide: A systematic review. BMC Complement. Med. Ther. 2020;20:363. doi: 10.1186/s12906-020-03157-2. - DOI - PMC - PubMed