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. 1985 Dec;6(6):757-68.
doi: 10.1007/BF00712240.

Thermodynamic analysis of calcium binding to frog parvalbumin

S J Smith, R C Woledge

Thermodynamic analysis of calcium binding to frog parvalbumin

S J Smith et al. J Muscle Res Cell Motil. 1985 Dec.

Abstract

The enthalpy of calcium binding to frog parvalbumin (Rana temporaria isoenzyme IVb, pI 4.75) has been measured by microcalorimetry. The reaction is exothermic; the heat of the reaction in 100 mM KCl, 50mM Tris, pH 8.0 at 12 degrees C is -19 kJ (mol site)-1 and -33 kJ (mol site)-1 in the presence of 1 mM magnesium. The shape of the titration curve indicates that the properties of the two calcium binding sites are different. The thermodynamic parameters measured for frog parvalbumin are compared with those of related parvalbumins from carp and whiting.

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References

    1. Biochim Biophys Acta. 1979 Jul 4;585(3):444-50 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. FEBS Lett. 1977;78(1):127-33 - PubMed
    1. FEBS Lett. 1974 Jun 1;42(2):173-8 - PubMed
    1. J Biol Chem. 1973 May 10;248(9):3313-26 - PubMed