Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 26;150(5):276.
doi: 10.1007/s00432-024-05804-4.

Upregulated lncRNA LINC01128 in colorectal cancer accelerates cell growth and predicts malignant prognosis through sponging miR-363-3p

Xiaohu Zhou #  1 , Yanhui Li #  2 , Lei Wu  1 , Chunyan Tian  3 , Xiaoliang Wu  4
Affiliations

Upregulated lncRNA LINC01128 in colorectal cancer accelerates cell growth and predicts malignant prognosis through sponging miR-363-3p

Xiaohu Zhou et al. J Cancer Res Clin Oncol. .

Abstract

Purpose: Colorectal cancer (CRC) refers to high-mortality tumors arising in the colon or rectum with a high rate of recurrence. The involvement of long non-coding RNAs (lncRNAs) contributes to the treatment and prognosis evaluation of CRC, and brings a new direction for the radical cure of patients. To identify the pathological mechanism and regulation of lncRNA LINC01128 (LINC01128) on CRC cells, and analyze its potential prognostic value.

Methods: LINC01128 level in tissue and cell specimens from 122 CRC patients was evaluated by RT-qPCR. The clinical significance and prognostic value of LINC01128 in CRC were analyzed via Kaplan-Meier and Cox analysis. CCK8 and Transwell assays were used to study the function of LINC01128 in vitro. The relationship between LINC01128 and miR-363-3p was confirmed by luciferase reporter gene assay.

Results: The overexpression of LINC01128 is associated with TNM stage and lymph node metastasis in CRC patients. Silencing LINC01128 inhibited the proliferation and metastasis of CRC cells. In addition, LINC01128 directly targeted and negatively regulated the miR-363-3p expression, while miR-363-3p inhibitor restored the inhibitory function of LINC01128.

Conclusion: As an independent prognostic factor of CRC, upregulation of LINC01128 predicts poor prognosis and accelerates tumor deterioration through miR-363-3p.

Keywords: Colorectal cancer; Invasion; Migration; Prognosis; lncRNA LINC01128; miR-363-3p.

PubMed Disclaimer

Conflict of interest statement

The authors have no relevant financial or non-financial interests to disclose.

Figures

Fig. 1
Fig. 1
Levels of LINC01128 and miR-363-3p in CRC samples. A, B LINC01128 and miR-363-3p in normal and CRC tissues. C, D LINC01128 and miR-363-3p in normal and CRC cells. ***P < 0.001
Fig. 2
Fig. 2
The prognostic value of LINC01128 in CRC patients. A Kaplan–Meier curve of LINC01128 with low and high expression (P = 0.004). B Probability of survival after recurrence in CRC patients (P = 0.001). C Forest plot of clinical indicators in CRC patients
Fig. 3
Fig. 3
Effect of LINC01128 knockdown on cell metastatic viability. A Transfection results of silenced LINC01128 in T84 and SNU-503 cells. B, C Cell proliferation was detected by CCK8 assay. Downregulation of LINC01128 suppressed the migration (D) and invasion (E) levels of T84 and SNU-503 cells. **P < 0.01, ***P < 0.001
Fig. 4
Fig. 4
Regulatory effect of LINC01128 on miR-363-3p. A There are binding sites between LINC01128 and miR-363-3p. B Determination of luciferase activity in WT-LINC01128 and MUT-LINC01128. C. Relative expression of miR-363-3p in T84 cells. D-E. Transfection of miR-363-3p inhibitor restored the inhibition of CRC cells by si-LINC01128. nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. &P < 0.05, &&&P < 0.001
See this image and copyright information in PMC

References

    1. Chen GQ, Liao ZM, Liu J, Li F, Huang D, Zhou YD (2021) LncRNA FTX promotes colorectal cancer cells migration and invasion by miRNA-590–5p/RBPJ axis. Biochem Genet 59:560–573. 10.1007/s10528-020-10017-8 - PubMed
    1. Deng Y et al (2022) Identification of EMT-related lncRNAs as potential prognostic biomarkers and therapeutic targets for pancreatic adenocarcinoma. J Oncol 2022:8259951. 10.1155/2022/8259951 - PMC - PubMed
    1. He A, Huang Z, Zhang R, Lu H, Wang J, Cao J, Feng Q (2022) Circadian clock genes are correlated with prognosis and immune cell infiltration in colon adenocarcinoma. Comput Math Methods Med 2022:1709918. 10.1155/2022/1709918 - PMC - PubMed
    1. Hu Y, Ma Y, Liu J, Cai Y, Zhang M, Fang X (2019) LINC01128 expedites cervical cancer progression by regulating miR-383–5p/SFN axis. BMC Cancer 19:1157. 10.1186/s12885-019-6326-5 - PMC - PubMed
    1. Li H et al (2020) LINC01128 resisted acute myeloid leukemia through regulating miR-4260/NR3C2. Cancer Biol Ther 21:615–622. 10.1080/15384047.2020.1740054 - PMC - PubMed