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. 2024 Sep;55(3):3031-3035.
doi: 10.1007/s42770-024-01395-x. Epub 2024 May 29.

ST88 ceftazidime-resistant Stenotrophomonas maltophilia is present in the saliva and gingival crevicular fluid of patients with periodontitis and AIDS from São Paulo State, Brazil

Felipe Pinheiro Vilela  1   2 , Amanda Akemi Kakumoto  2 , Carolina Nogueira Gomes  1 , Tábata Larissa Santos Pólvora  2 , Átila Vinícius Vítor Nobre  2 , Alan Grupioni Lourenço  2 , Ana Carolina Fragoso Motta  2 , Juliana Pfrimer Falcão  3
Affiliations

ST88 ceftazidime-resistant Stenotrophomonas maltophilia is present in the saliva and gingival crevicular fluid of patients with periodontitis and AIDS from São Paulo State, Brazil

Felipe Pinheiro Vilela et al. Braz J Microbiol. 2024 Sep.

Abstract

Stenotrophomonas maltophilia (S. maltophilia) is an intrinsically drug-resistant and biofilm-forming bacteria causing infections in immunocompromised humans. This study reports the isolation of five S. maltophilia strains from saliva and gingival crevicular fluid (GCF) of AIDS patients with periodontitis in São Paulo, Brazil, showing resistance to ceftazidime, strong biofilm formation capacity and a close genetic relationship. The presence of S. maltophilia strains in saliva and CGF of patients with AIDS and periodontitis is a concern for the presence and persistence of intrinsically resistant bacteria in the oral environment, enhancing the risk for the development of severe infections in immunocompromised patients.

Keywords: Stenotrophomonas maltophilia; Antibiotic resistance; HIV; Molecular typing; biofilms.

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Conflict of interest statement

Juliana Pfrimer Falcão is an Associated Editor of the Brazilian Journal of Microbiology, and the peer review process for this article was independently handled by another member of the journal’s editorial board. No other financial or non-financial conflict of interest is declared by the authors.

Figures

Fig. 1
Fig. 1
16S rRNA phylogeny (A), Pulsed-Field Gel Electrophoresis (B), Enterobacterial Repetitive Intergenic Consensus - PCR (C) and Multi-Locus Sequence Typing (D) analyses of Stenotrophomonas maltophilia (S. maltophilia) strains isolated from the saliva (n = 1) and gingival crevicular fluid (n = 4) of HIV + patients with AIDS and chronic periodontitis from the state of São Paulo, Brazil. The 16S rRNA phylogenetic analysis (A) was constructed in MEGA11, using the MUSCLE for the alignment, and the Bootstrap method with 1000 replicates for phylogeny inference to build a Maximum Parsimony tree. Six additional S. maltophilia sequences were included for comparison, and Escherichia coli U 5/41 an outgroup. XbaI PFGE (B) and ERIC-PCR (C) similarity dendrograms were created with Bionumerics 7.6, using the UPGMA method, the Dice similarity coefficient and a 1% tolerance. Red and blue squares represent strains isolated from gingival crevicular fluid and saliva, respectively. The minimum spanning tree constructed for MLST (D) was built with PHILOViZ and included additional 835 sequence types (STs) of 1,177 S. maltophilia strains publicly available at PubMLST. The size difference among nodes are related to the amount of strains belonging to each ST. ST88 is highlighted in red, and a closer look to its closely related STs is shown
See this image and copyright information in PMC

References

    1. Brooke JS (2012) Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clin Microbiol Rev 25(1):2–41. 10.1128/CMR.00019-11 10.1128/CMR.00019-11 - DOI - PMC - PubMed
    1. Soga Y, Saito T, Nishimura F et al (2008) Appearance of multidrug-resistant opportunistic bacteria on the gingiva during leukemia treatment. J Periodontol 79(1):181–186. 10.1902/jop.2008.070205 10.1902/jop.2008.070205 - DOI - PubMed
    1. Prates M, Fernandes F, Proença F et al (2020) Oral infection caused by Stenotrophomonas maltophilia: a rare presentation of an Emerging Opportunistic Pathogen. Case Rep Infect Dis 2020(6346120). 10.1155/2020/6346120 - PMC - PubMed
    1. Aitken SL, Sahasrabhojane PV, Kontoyiannis DP et al (2021) Alterations of the oral Microbiome and cumulative Carbapenem exposure are Associated with Stenotrophomonas maltophilia infection in patients with Acute myeloid leukemia receiving chemotherapy. Clin Infect Dis 72(9):1507–1513. 10.1093/cid/ciaa778 10.1093/cid/ciaa778 - DOI - PMC - PubMed
    1. Brito LC, Sobrinho AP, Teles RP et al (2012) Microbiologic profile of endodontic infections from HIV- and HIV + patients using multiple-displacement amplification and checkerboard DNA-DNA hybridization. Oral Dis 18(6):558–567. 10.1111/j.1601-0825.2012.01908.x 10.1111/j.1601-0825.2012.01908.x - DOI - PMC - PubMed

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