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. 2024 May 1;25(5):1815-1821.
doi: 10.31557/APJCP.2024.25.5.1815.

Anticancer Activity of Aaptos suberitoides on Glioblastoma Multiforme Cell Line

Fathul Huda  1 , Rohim Hermawan  2 , Tenny Putri  3 , Resti Gradia Dwiwina  1 , Afiat Berbudi  1 , Muhammad Hasan Bashari  1
Affiliations

Anticancer Activity of Aaptos suberitoides on Glioblastoma Multiforme Cell Line

Fathul Huda et al. Asian Pac J Cancer Prev. .

Abstract

Objective: Glioblastoma Multiforme (GBM) poses a significant challenge due to its high aggressiveness and unfavorable prognosis, with existing treatments demonstrating limited efficacy in prolonging survival rates. This study aimed to assess the anticancer properties of Aaptos suberitoides extracts and fraction on the U87 cell line, serving as a representative model for GBM.

Methods: U87 cells were treated with ethanol extracts derived from Aaptos suberitoides, specifically two extracts (OAA-1 and OAA-2) and one ethyl acetate fraction (EA) isolated from specimens collected on Pramuka Island and Tinjil Island. The evaluation encompased microscopic observation and MTT assay to determine the IC50. Subsequently, antiproliferative effects were investigated through apoptosis and cell cycle assays.

Results: The extract demonstrated cytotoxic activity against U87 cells, with OAA-1 and OAA-2 exhibiting IC50 values of 35.78 μg/mL and 25.38 μg/mL, respectively. OAA-1 notably induced apoptosis at 50 μg/mL and induced cell cycle arrest. On other hand, OAA-2, while also inducing apoptosis significantly, had a lesser impact on cell cycle arrest. In contrast, EA induced significant apoptosis at a concentration of 100 μg/mL.

Conclusion: The ethanol extracts and the ethyl acetate fraction of Aaptos suberitoides emerged as a promising candidate for Glioblastoma Multiforme cancer therapy, showing potential in inhibiting cell proliferation and inducing apoptosis.

Keywords: Aaptos suberitoides; Antineoplastic agents; Glioblastoma; Marine Substance; Phytogenic.

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Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

Figure 1
Figure 1
Cytotoxic Curve and Microscopic (magnification 100x) Appearance of U87 Cell by Treatment of extract of Aaptos suberitoides. (a) Curve of percentage of cells death and dose of treatment. (b) Microscopic appearance of U87 cell after 72-h treatment.
Figure 2
Figure 2
Apoptosis Analysis Using Flowcytometer. (a) Statistical analysis of Aaptos suberitoides inducing apoptosis on U87 cels with AnnexinV/PI marker. (b) Pseudocolor density plot of U87
Figure 3
Figure 3
Cell Cycle Analysis Using Flowcytometry. (a) Statististical analysis of U87 cell line and (b) Cell cycle curve of U87
See this image and copyright information in PMC

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