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. 2024 Jun 6;84(11):2053-2069.e9.
doi: 10.1016/j.molcel.2024.05.003. Epub 2024 May 28.

FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo

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Free article

FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo

Kristina Žumer et al. Mol Cell. .
Free article

Abstract

Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze the in vivo functions of FACT with the use of multi-omics analysis after rapid FACT depletion from human cells. We show that FACT depletion destabilizes chromatin and leads to transcriptional defects, including defective promoter-proximal pausing and elongation, and increased premature termination of RNA polymerase II. Unexpectedly, our analysis revealed that promoter-proximal pausing depends not only on the negative elongation factor (NELF) but also on the +1 nucleosome, which is maintained by FACT.

Keywords: FACT; RNA polymerase II; chromatin; nucleosome; promoter-proximal pausing; transcription.

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Conflict of interest statement

Declaration of interests The authors declare no competing interests.

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