FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo
- PMID: 38810649
- DOI: 10.1016/j.molcel.2024.05.003
FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo
Abstract
Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. Here, we analyze the in vivo functions of FACT with the use of multi-omics analysis after rapid FACT depletion from human cells. We show that FACT depletion destabilizes chromatin and leads to transcriptional defects, including defective promoter-proximal pausing and elongation, and increased premature termination of RNA polymerase II. Unexpectedly, our analysis revealed that promoter-proximal pausing depends not only on the negative elongation factor (NELF) but also on the +1 nucleosome, which is maintained by FACT.
Keywords: FACT; RNA polymerase II; chromatin; nucleosome; promoter-proximal pausing; transcription.
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of interests The authors declare no competing interests.
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