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. 2024 May 15:11:1388173.
doi: 10.3389/fvets.2024.1388173. eCollection 2024.

Effects of different addition levels of CHM-JM113 on growth performance, antioxidant capacity, organ index, and intestinal health of AA broilers

Guanhua Fu #  1 , Mengyu Zhang #  1 , Yuanyuan Huang  1   2 , Runyu Han  1 , Kaixuan Qi  1 , Lidong Yin  1 , Dongchen Zhao  1 , Yueyan Huang  1 , Tenghe Ma  1 , Lihong Wang  1
Affiliations

Effects of different addition levels of CHM-JM113 on growth performance, antioxidant capacity, organ index, and intestinal health of AA broilers

Guanhua Fu et al. Front Vet Sci. .

Abstract

The purpose of the present study was to investigate the effects of different levels of a Chinese herbal medicine formulation combined with JM113 (CHM-JM113) on growth performance, antioxidant capacity, organ index, and intestinal health of AA broilers. The AA broiler chicks were randomly allocated to 5 treatments as follows: a basic diet for the control group, the basic diet supplemented with 0.25% CHM-JM113, 0.5% CHM-JM113, 1% CHM-JM113 and 2% CHM-JM113 for the treatment group, respectively. The results showed that the addition of CHM-JM113 to the diet significantly reduced the mortality (p < 0.01) and improved the European Broiler Index (EBI) (p < 0.05), whereas it had no significance on growth performance of AA broilers (p > 0.05). Comparing the control group, 0.5 and 1% CHM-JM113 group significantly improved the organ index of liver, spleen and bursa (p < 0.05). In terms of intestinal morphology and structure, the addition of different levels of CHM-JM113 increased VH and VH/CD ratio, decreased CD in the small intestine compared to the control group, with 1 and 2% of the additive dose being more effective (p < 0.05). Chinese herbal medicine and probiotics as natural antioxidants also significantly increased the content of SOD in serum of 21-day-old broilers (p < 0.01), and significantly decreased the content of MDA in serum (p < 0.01). At 42 days of age, the addition of 1 and 2% CHM-JM113 significantly increased the content of SOD (p < 0.01) and significantly decreased the content of MDA in the organism (p < 0.01), accompanied by a significant increase in T-AOC and CAT content. In the study of the effect of CHM-JM113 on intestinal immunity, compared with the control group, we found that 1% or 2% CHM-JM113 had a better effect on the expression of occludin and claudin-1 in the intestinal segments of broilers (p < 0.05). For the expression of GATA-3, 0.5% CHM-JM113 may have a better effect (p < 0.05). CHM-JM113 may be used as an antibiotic alternative in broiler production.

Keywords: AA broiler; Chinese herbal medicine; JM113; antioxidant capacity; growth performance; intestinal health; organ index.

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Conflict of interest statement

YuaH was employed by Muyuan Foods Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The duodenum, jejunum and ileum histological morphology of 21 days of broilers (hematoxylin and eosin). CON, broilers were fed with a basal diet; 0.25, 0.5, 1 and 2, broilers were fed with a basal diet supplemented with 0.25, 0.5, 1 and 2% CHM-JM113, respectively. Scale bars = 200 μm.
Figure 2
Figure 2
The duodenum, jejunum and ileum histological morphology of 42 days of broilers (hematoxylin and eosin). CON, broilers were fed with a basal diet; 0.25, 0.5, 1 and 2, broilers were fed with a basal diet supplemented with 0.25, 0.5, 1 and 2% CHM-JM113, respectively. Scale bars = 200 μm.
Figure 3
Figure 3
Effects of CHM-JM113 on gene expression levels of occludin, claudin-1 and GATA-3 in duodenum of broilers (day 21).
Figure 4
Figure 4
Effects of CHM-JM113 on gene expression levels of occludin, claudin-1 and GATA-3 in duodenum of broilers (day 42).
Figure 5
Figure 5
Effects of CHM-JM113 on gene expression levels of occludin, claudin-1 and GATA-3 in jejunum of broilers (day 21).
Figure 6
Figure 6
Effects of CHM-JM113 on gene expression levels of occludin, claudin-1 and GATA-3 in jejunum of broilers (day 42).
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