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. 1985 Jan;161(1):442-5.
doi: 10.1128/jb.161.1.442-445.1985.

Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains

P R MacLachlan, K E Sanderson

Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains

P R MacLachlan et al. J Bacteriol. 1985 Jan.

Abstract

Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.

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