Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Jan;161(1):442-5.
doi: 10.1128/jb.161.1.442-445.1985.

Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains

P R MacLachlan, K E Sanderson

Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains

P R MacLachlan et al. J Bacteriol. 1985 Jan.

Abstract

Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequencies 8- to 630-fold higher than with smooth strains or other rough mutants.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Bacteriol. 1979 Aug;139(2):376-83 - PubMed
    1. Microbiol Rev. 1983 Sep;47(3):410-53 - PubMed
    1. J Gen Microbiol. 1980 Jan;116(1):25-32 - PubMed
    1. J Bacteriol. 1974 Feb;117(2):406-16 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed

Publication types

MeSH terms

LinkOut - more resources