Separate resistances to arsenate and arsenite (antimonate) encoded by the arsenical resistance operon of R factor R773
- PMID: 3881408
- PMCID: PMC214947
- DOI: 10.1128/jb.161.2.758-763.1985
Separate resistances to arsenate and arsenite (antimonate) encoded by the arsenical resistance operon of R factor R773
Abstract
The arsenical resistance operon of R factor R773 was analyzed by subcloning and insertional inactivation. The operon was found to have two functional regions, the promoter-proximal region encoding resistance to arsenite and antimonate and the promoter-distal region encoding arsenate resistance. A unique 1.6-kilobase fragment was shown to be sufficient to encode arsenate resistance and produce arsenate extrusion from intact cells.
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