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Review
. 2024 May 31;15(1):155.
doi: 10.1186/s13287-024-03768-3.

Current applications of intestinal organoids: a review

Tao Xiang  1 , Jie Wang  2 , Hui Li  3
Affiliations
Review

Current applications of intestinal organoids: a review

Tao Xiang et al. Stem Cell Res Ther. .

Abstract

In the past decade, intestinal organoid technology has paved the way for reproducing tissue or organ morphogenesis during intestinal physiological processes in vitro and studying the pathogenesis of various intestinal diseases. Intestinal organoids are favored in drug screening due to their ability for high-throughput in vitro cultivation and their closer resemblance to patient genetic characteristics. Furthermore, as disease models, intestinal organoids find wide applications in screening diagnostic markers, identifying therapeutic targets, and exploring epigenetic mechanisms of diseases. Additionally, as a transplantable cellular system, organoids have played a significant role in the reconstruction of damaged epithelium in conditions such as ulcerative colitis and short bowel syndrome, as well as in intestinal material exchange and metabolic function restoration. The rise of interdisciplinary approaches, including organoid-on-chip technology, genome editing techniques, and microfluidics, has greatly accelerated the development of organoids. In this review, VOSviewer software is used to visualize hot co-cited journal and keywords trends of intestinal organoid firstly. Subsequently, we have summarized the current applications of intestinal organoid technology in disease modeling, drug screening, and regenerative medicine. This will deepen our understanding of intestinal organoids and further explore the physiological mechanisms of the intestine and drug development for intestinal diseases.

Keywords: Clinical trail; Disease; Drug-screening; Inflammatory bowel disease; Intestinal organoid; Intestinal transplantation.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Applications of intestinal organoids. The various applications of intestinal organoids are as follows: (1) Developing in vitro models of intestinal organoids derived from adult tissues, pluripotent stem cells, or embryonic stem cells. (2) Constructing organoid from healthy individuals or patients for biobanks and drug screening. (3) Utilizing organoids for transplantation in the treatment of intestinal diseases. (4) Engineering modified organoids through interdisciplinary collaboration. iPSC induced pluripotent stem cell, ESC embryonic stem cell, IBD inflammatory bowel disease. Created with BioRender.com
Fig. 2
Fig. 2
Co-citation analysis of intestinal organoids. The journals in the VOSviewer software are identified 674 journals, including 297 key journals. Created with VOSviewer software
Fig. 3
Fig. 3
Co-word analysis of intestinal organoids. The keywords in the VOSviewer software are divided into 6 topic clusters. Created with VOSviewer software
See this image and copyright information in PMC

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