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. 2024 Jul:33:77-79.
doi: 10.1016/j.jtos.2024.05.003. Epub 2024 May 31.

A novel severity scoring system for murine ocular graft versus host disease and its correlation with CD3+ T cells in the cornea

Fernando Martinez Guasch  1 , Seema Sajjan  1 , Ellis Tibbs  2 , Cassidy Reandeau  1 , Long Wu  2 , Jerry Bohlen  1 , Andrew Li  1 , Amy Plotkin  1 , Xuefang Cao  2 , Sarah B Sunshine  3
Affiliations

A novel severity scoring system for murine ocular graft versus host disease and its correlation with CD3+ T cells in the cornea

Fernando Martinez Guasch et al. Ocul Surf. 2024 Jul.
No abstract available

Keywords: Cornea; GVHD; Mouse model; Ocular graft versus host disease.

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Conflict of interest statement

Declaration of competing interest None.

Figures

Fig. 1.
Fig. 1.
A) Ocular graft versus host disease (oGVHD) grading scale incorporating corneal staining, eyelid inflammation, eyelid fur loss (black arrow demonstrates patch of alopecia on superior eyelid), and corneal haze (blue arrows demonstrate reflection of nose cone used during capture). B) Ocular and C) Systemic GVHD scoring by week in splenocyte groups 1×106 (N = 4), 2×106 (N = 4), 5×106 (N = 4), control (N = 3) D) Linear regression of average oGVHD scores on day 40 compared to average CD3+ cell counts on day 40 by study group. E) CD3+ immunohistochemistry (IHC) cells in central cornea of control mouse (0.07 ± 0.33). F) CD3+ IHC cells in central cornea in 1×106 splenocytes mouse (0.13 ± 0.40). G) CD3+ IHC staining in central cornea in 2×106 splenocytes mouse (0.70 ± 0.96). H) CD3+ IHC cells in central cornea in 5×106 splenocytes mouse (6.88 ± 4.57). I) CD3+ IHC cells in peripheral cornea in control mouse (2.30 ± 2.30). J) CD3+ IHC staining in peripheral cornea in 1×106 splenocytes mouse (0.98 ± 1.67). K) CD3+ IHC cells in peripheral cornea in 2×106 splenocytes mouse (8.75 ± 10.10). L) CD3+ IHC cells in peripheral cornea in 5×106 splenocytes mouse (22.44 ± 12.60).
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