Light drives nitrogen fixation in tropical montane cloud forests in Costa Rica

Abstract

Tropical montane cloud forests are high altitude ecosystems characterized by very high ambient humidity, which favors organisms that depend on the environment for their water status, such as bryophytes and their nitrogen-fixing symbionts. Bryophyte-associated N₂ fixation is a major source of new N in several northern environments, but their contributions to the N cycle in other ecosystems is still poorly understood. In this work, we evaluated N₂ fixation rates associated with epiphytic bryophytes growing along the stems of pumpwood trees (Cecropia sp.) as well as in surrounding litter and soil from a primary and a secondary cloud forests in the Talamanca Mountain Range, Costa Rica. Nitrogen fixation was significantly higher in substrates from the secondary forest compared to those from the primary forest. Overall, N₂ fixation rates associated with epiphytic bryophytes were 57 times those of litter and 270 times what was measured in soil. Further, light intensity was the major factor influencing N₂ fixation rates in all substrates. Increased access to light in disturbed cloud forests may therefore favor bryophyte-associated N₂ fixation, potentially contributing to the recovery of these ecosystems.

Keywords: Bryophyte; Cyanobacteria; Epiphyte; Litter; Soil; Tropical forest.