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. 1985 Feb 25;181(2):407-11.
doi: 10.1016/0014-5793(85)80302-1.

Convenient modification of the method for oligonucleotide-directed in vitro mutagenesis of cloned DNA

V A Efimov, O V Mirskikh, O G Chakhmakhcheva, Ovchinnikov YuA
Free article

Convenient modification of the method for oligonucleotide-directed in vitro mutagenesis of cloned DNA

V A Efimov et al. FEBS Lett. .
Free article

Abstract

A new modification of the oligonucleotide-mediated mutagenesis technique has been developed. The proposed methodology has been used to produce specific base changes in the double-stranded plasmid DNA. For this purpose, special cloning vectors have been constructed using the synthetic oligodeoxyribonucleotides. The developed method allows the production of mutant DNA from those of the wild-type with a yield of 10-20%.

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