Passage of Salmonella enteritidis and Salmonella thompson through chick ileocecal mucosa

Abstract

The passage of Salmonella enteritidis and S. thompson across the cecal mucosa has been visualized in an electron microscope study with the freshly hatched chick as a model. The uptake of salmonellae by macrophages took place in the cecal lumen; the macrophages became abnormal and often ruptured to release organisms back into the lumen. The entry of bacteria into the epithelial cells was associated with a series of pathological changes, beginning with the appearance of active Golgi apparatus and the production of a variety of lysosomal vesicles. Salmonellae became sequestered within lysosomes but were unaffected by the presence of hydrolytic enzyme. Epithelial cell death was related to particularly large numbers of bacteria. Fragments of invaded epithelial cells, especially those undergoing cell death, contributed to the cytoplasmic debris and released further salmonellae into the lumen. Bacteria were never observed in large numbers below the basement membrane, and there was no significant pathology in the lamina propria tissue. Wandering cells, identified as macrophages and containing the bacteria, were observed spanning the epithelial and lamina propria regions through breaks in the basement membrane. It is suggested that the passage of bacteria from the epithelium to the lamina propria is primarily the result of capture and transport within host macrophages.