Growth of murine bone marrow adherent stromal cells in culture without hydrocortisone in a low oxygen environment

Abstract

Successful culture of hematopoietic cells depends upon the growth of stromal cells. The growth of bone marrow stromal cells is best achieved when hydrocortisone is added to the culture medium. However, the hydrocortisone requirement is eliminated if the cultures are grown in an atmosphere of 5% CO2, 5% O2, and 90% N2 rather than 5% CO2 in air, which is more common.