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. 2024 Jun 3;51(1):722.
doi: 10.1007/s11033-024-09561-y.

Screening and identification of DNA nucleic acid aptamers against F1 protein of Yersinia pestis using SELEX method

Nafiseh Shafiei  1 , Hamideh Mahmoodzadeh Hosseini  2 , Jafar Amani  3 , Seyed Ali Mirhosseini  3 , Hanieh Jafary  1
Affiliations

Screening and identification of DNA nucleic acid aptamers against F1 protein of Yersinia pestis using SELEX method

Nafiseh Shafiei et al. Mol Biol Rep. .

Abstract

Background: Yersinia pestis is a bacterium that causes the disease plague. It has caused the deaths of many people throughout history. The bacterium possesses several virulence factors (pPla, pFra, and PYV). PFra plasmid encodes fraction 1 (F1) capsular antigen. F1 protein protects the bacterium against host immune cells through phagocytosis process. This protein is specific for Y. pestis. Many diagnostic techniques are based on molecular and serological detection and quantification of F1 protein in different food and clinical samples. Aptamers are small nucleic acid sequences that can act as specific ligands for many targets.This study, aimed to isolate the high-affinity ssDNA aptamers against F1 protein.

Methods and results: In this study, SELEX was used as the main strategy in screening aptamers. Moreover, enzyme-linked aptamer sorbent assay (ELASA) and surface plasmon resonance (SPR) were used to determine the affinity and specificity of obtained aptamers to F1 protein. The analysis showed that among the obtained aptamers, the three aptamers of Yer 21, Yer 24, and Yer 25 were selected with a KD value of 1.344E - 7, 2.004E - 8, and 1.68E - 8 M, respectively. The limit of detection (LoD) was found to be 0.05, 0.076, and 0.033 μg/ml for Yer 21, Yer 24, and Yer 25, respectively.

Conclusion: This study demonstrated that the synthesized aptamers could serve as effective tools for detecting and analyzing the F1 protein, indicating their potential value in future diagnostic applications.

Keywords: Yersinia Pestis; DNA aptamer; ELASA; SELEX; SPR.

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References

    1. Hussein MF (2021) Yersinia pestis. Infectious diseases of dromedary camels: a concise guide. Springer, pp 201–204 - DOI
    1. Dheyab ZS (2022) Clinically important Yersinia: minireview. J Med Health Sci 5:2295–2306
    1. Demeure CE, Dussurget O, Mas Fiol G, Le Guern A-S, Savin C, Pizarro-Cerdá J (2019) Yersinia pestis and plague: an updated view on evolution, virulence determinants, immune subversion, vaccination, and diagnostics. Genes Immun 20:357–370. https://doi.org/10.1038/s41435-019-0065-0 - DOI - PubMed - PMC
    1. Sebbane F, Uversky VN, Anisimov AP (2020) Yersinia pestis plasminogen activator. Biomolecules 10:1554. https://doi.org/10.3390/biom10111554 - DOI - PubMed - PMC
    1. Rosario-Acevedo R, Biryukov SS, Bozue JA, Cote CK (2021) Plague prevention and therapy: perspectives on current and future strategies. Biomedicines 9:1421. https://doi.org/10.3390/biomedicines9101421 - DOI - PubMed - PMC

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