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. 2024 May 30:19:5045-5056.
doi: 10.2147/IJN.S455936. eCollection 2024.

Chemodynamic PtMn Nanocubes for Effective Photothermal ROS Storm a Key Anti-Tumor Therapy in-vivo

Chen Wang #  1 , Hongmei Zhou #  1 , Mekhrdod S Kurboniyon  2 , Yanping Tang  1 , Zhengmin Cai  1 , Shufang Ning  1 , Litu Zhang  1 , Xinqiang Liang  1
Affiliations

Chemodynamic PtMn Nanocubes for Effective Photothermal ROS Storm a Key Anti-Tumor Therapy in-vivo

Chen Wang et al. Int J Nanomedicine. .

Abstract

Background: Chemodynamic therapy (CDT) is a new treatment approach that is triggered by endogenous stimuli in specific intracellular conditions for generating hydroxyl radicals. However, the efficiency of CDT is severely limited by Fenton reaction agents and harsh reaction conditions.

Methods: Bimetallic PtMn nanocubes were rationally designed and simply synthesized through a one-step high-temperature pyrolysis process by controlling both the nucleation process and the subsequent crystal growth stage. The polyethylene glycol was modified to enhance biocompatibility.

Results: Benefiting from the alloying of Pt nanocubes with Mn doping, the structure of the electron cloud has changed, resulting in different degrees of the shift in electron binding energy, resulting in the increasing of Fenton reaction activity. The PtMn nanocubes could catalyze endogenous hydrogen peroxide to toxic hydroxyl radicals in mild acid. Meanwhile, the intrinsic glutathione (GSH) depletion activity of PtMn nanocubes consumed GSH with the assistance of Mn3+/Mn2+. Upon 808 nm laser irradiation, mild temperature due to the surface plasmon resonance effect of Pt metal can also enhance the Fenton reaction.

Conclusion: PtMn nanocubes can not only destroy the antioxidant system via efficient reactive oxygen species generation and continuous GSH consumption but also propose the photothermal effect of noble metal for enhanced Fenton reaction activity.

Keywords: Fenton reaction; Mn-doping; chemodynamic therapy; noble metal; photothermal effect.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
(a and b) TEM images of PtMn nanocubes. (c) SAED pattern of PtMn nanocubes. (d and e) Mn and Pt element mappings of PtMn nanocubes. (f) XRD pattern of PtMn nanocubes.
Figure 2
Figure 2
(a) UV-vis absorption spectra of oxTMB catalyzed by PtMn nanocubes under different pH conditions. (b) UV-vis absorption spectra of oxTMB catalyzed by PtMn nanocubes in different groups. (c) UV-vis absorption spectra of oxTMB catalyzed by PtMn nanocubes with time increase. (d) The color photo of oxTMB catalyzed by PtMn nanocubes. (e) Michaelis-Menten curves and (f) Lineweaver-Burk plots of Pt and PtMn nanocubes. Data presented as mean ± standard deviation (n = 3). (g) ESR spectra of ·OH in PtMn+H2O2 and Pt+H2O2 nanocubes. (h) UV-vis absorption spectra of DTNB catalyzed by Pt and PtMn nanocubes. (i) Quantitative analysis of (h).
Figure 3
Figure 3
(a) The photothermal effect of PtMn nanocubes with different concentrations. (b) The temperature increase of PtMn nanocubes under different laser powers. (c) Infrared thermal imaging of PtMn nanocubes under different concentrations with time increase. (d) The photothermal conversion efficiency of PtMn nanocubes. (e) The photothermal stability of PtMn nanocubes.
Figure 4
Figure 4
(a) Cellular uptake performance of RhB-label PtMn nanocubes. (b and c) MTT assay of PtMn nanocubes on L929 and Huh7 cells, respectively, for biocompatibility and cytotoxicity estimation. (d) ROS generation, GSH depletion, O2 generation abilities of PtMn nanocubes, and live/dead cell staining.
Figure 5
Figure 5
(a) Biodistribution of Pt in main organs and tumors. (b) Tumor volume and (c) tumor weight in different groups. Data presented as mean ± S.D. (n = 5). (d) The body weight of mice in different groups. Data presented as mean ± S.D. (n = 5). (e) H&E-staining assay of tumor tissues.
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