Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 May 31:17:2199-2211.
doi: 10.2147/IDR.S456961. eCollection 2024.

Genotypes and Phylogenetic Analysis of Human Adenovirus in Hospitalized Pneumonia and Influenza-Like Illness Patients in Jiangsu Province, China (2013-2021)

Shenjiao Wang #  1   2 , Xin Zou #  3   4   5 , Jianguang Fu  1 , Fei Deng  1 , Huiyan Yu  1 , Huan Fan  1 , Qigang Dai  1 , Qingxiang Shang  5 , Ke Xu  1 , Changjun Bao  1   5
Affiliations

Genotypes and Phylogenetic Analysis of Human Adenovirus in Hospitalized Pneumonia and Influenza-Like Illness Patients in Jiangsu Province, China (2013-2021)

Shenjiao Wang et al. Infect Drug Resist. .

Abstract

Background: Human adenovirus (HAdV) is common pathogens that cause various respiratory diseases. The genetic diversity of viruses caused by recombination is considered to be the main source of emerging outbreaks. The aim of this study is to explore the evolutionary relationship and recombination events of HAdV genome in respiratory tract infections in Jiangsu Province.

Methods: Whole-genome sequencing (WGS) technology was used to sequence 66 patients with HAdV infection (37 patients with influenza-like illness (ILI) and 29 hospitalized patients with pneumonia) from Jiangsu Province. Epidemiological analysis was performed on hospitalized pneumonia and ILI patients infected with HAdV. Subsequently, phylogenetic, recombination, and nucleotide and amino acid identity analyses were performed.

Results: Epidemiological analysis of patients undergoing WGS showed that 75.7% of ILI patients were infected with the HAdVB strain and 69.0% of hospitalized pneumonia patients were infected with the HAdVC strain. Moreover, the hospitalized pneumonia and ILI patients infected with HAdV were different in region and time. The strains of HAdVB3 and HAdVB7 genotypes were mainly infected in 2015 and 2017, and the strains of HAdVC1 and HAdVC2 genotypes were mainly infected in 2020. The results of histogram analysis showed that the HAdV strain mainly infected children under 5 years old. In addition, 36 novel recombinant strains were identified. The discovery of these recombinant strains may contribute to understanding the epidemiology of HAdV and research on related vaccines. Furthermore, the percentage of nucleotide and amino acid identities revealed a high level of genetic conservation within isolates from HAdVB3, HAdVB7, HAdVC1, HAdVC2 and HAdVC5 genotypes.

Conclusion: The WGS analysis reveals the evolutionary relationships and recombination events of HAdV strains in Jiangsu Province, which is helpful to deepen the understanding of HAdV epidemiology and evolution. In addition, it provides a basis for the formulation of public health strategies in Jiangsu Province.

Keywords: human adenovirus; phylogenetic analysis; recombination; whole genome sequencing.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
The regional distribution, year, and genotypes of infection of HAdV infected patients in this study. (A) The regional distribution of HAdV infected patients in this study; (B) The year of infection of HAdV infected patients in this study; (C) The genotype distribution for different years; (D) The genotype distribution for different ages.
Figure 2
Figure 2
Phylogenetic analysis of all HAdV strains detected in this study based on WGS data. Red stars represent hospitalized pneumonia patients, and blue circles represent ILI patients.
Figure 3
Figure 3
Phylogenetic analysis of HAdVB. (A) The phylogenetic analysis of whole genome; (B) The phylogenetic analyses of hexon genes from species HAdVB; (C) The phylogenetic analyses of penton genes from species HAdVB; (D) The phylogenetic analyses of fiber genes from species HAdVB. Red stars represent hospitalized pneumonia patients, and blue circles represent ILI patients.
Figure 4
Figure 4
Phylogenetic analysis of HAdVC. (A) The phylogenetic analysis of whole genome; (B) The phylogenetic analyses of hexon genes from species HAdVC; (C) The phylogenetic analyses of penton genes from species HAdVC; (D) The phylogenetic analyses of fiber genes from species HAdVC. Red stars represent hospitalized pneumonia patients, and blue circles represent ILI patients.
Figure 5
Figure 5
Phylogenetic analysis of HAdVE. (A) The phylogenetic analysis of whole genome; (B) The phylogenetic analyses of hexon genes from species HAdVE; (C) The phylogenetic analyses of penton genes from species HAdVE; (D) The phylogenetic analyses of fiber genes from species HAdVE. Blue circles represent ILI patients.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Punga T, Darweesh M, Synthesis AG. Structure, and Function of Human Adenovirus Small Non-Coding RNAs. Viruses. 2020;12(10):1182. doi:10.3390/v12101182 - DOI - PMC - PubMed
    1. Davison AJ, Benkő M, Harrach B. Genetic content and evolution of adenoviruses. J Gen Virol. 2003;84(Pt 11):2895–2908. doi:10.1099/vir.0.19497-0 - DOI - PubMed
    1. Nemerow GR, Stewart PL, Reddy VS. Structure of human adenovirus. Curr Opin Virol. 2012;2(2):115–121. doi:10.1016/j.coviro.2011.12.008 - DOI - PMC - PubMed
    1. Tamanini A, Nicolis E, Bonizzato A, et al. Interaction of adenovirus type 5 fiber with the coxsackievirus and adenovirus receptor activates inflammatory response in human respiratory cells. J Virol. 2006;80(22):11241–11254. doi:10.1128/JVI.00721-06 - DOI - PMC - PubMed
    1. Mennechet FJD, Paris O, Ouoba AR, et al. A review of 65 years of human adenovirus seroprevalence. Jun. 2019;18(6):597–613. - PubMed