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. 1979 Nov 24;7(6):1593-601.
doi: 10.1093/nar/7.6.1593.

Modified polynucleotides. IV. Template activity of 5-alkyluracil-containing poly [d(A-r5U)] copolymers for DNA and RNA polymerases

J Sági, L Otvös
Free PMC article

Modified polynucleotides. IV. Template activity of 5-alkyluracil-containing poly [d(A-r5U)] copolymers for DNA and RNA polymerases

J Sági et al. Nucleic Acids Res. .
Free PMC article

Abstract

The change of templating properties for E. coli DNA and RNA polymerases of poly[d(A-r5U)] copolymers on substitution of the uracil for 5-methyl-, 5-ethyl-, 5-n-propyl-, 5-n-butyl- or 5-n-pentyluracil, resp., was studied. All modified polymers proved to be more effective template-primers than poly[d(A-U)] or poly[d(A-T)] for the DNA polymerase. Direct dependence of the template-primer efficiency (vmax/Km) value on the thermal stability of the polymers was observed. For transcription the r = ethyl and n-propyl polymers were also more effective templates than poly[d(A-T)].

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References

    1. Nucleic Acids Res. 1977 Aug;4(8):2767-77 - PubMed
    1. Nucleic Acids Res. 1979 Jun 25;6(8):2839-48 - PubMed
    1. Biochemistry. 1973 Jan 2;12(1):151-7 - PubMed

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