Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Feb 15;226(1):217-23.
doi: 10.1042/bj2260217.

The purification of shikimate dehydrogenase from Escherichia coli

S Chaudhuri, J R Coggins

The purification of shikimate dehydrogenase from Escherichia coli

S Chaudhuri et al. Biochem J. .

Abstract

A procedure was developed for the purification of shikimate dehydrogenase from Escherichia coli. Homogeneous enzyme with specific activity 1100 units/mg of protein was obtained in 21% overall yield. The subunit Mr estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 32 000. The native Mr, estimated by gel-permeation chromatography on a TSK G2000SW column, was also 32 000. E. coli shikimate dehydrogenase is therefore a monomeric NADP-linked dehydrogenase.

PubMed Disclaimer

References

    1. J Bacteriol. 1966 Apr;91(4):1494-508 - PubMed
    1. Nature. 1979 Feb 8;277(5696):486-9 - PubMed
    1. J Bacteriol. 1969 Jul;99(1):222-30 - PubMed
    1. J Bacteriol. 1979 Jun;138(3):933-43 - PubMed
    1. J Biol Chem. 1982 Feb 10;257(3):1491-500 - PubMed

MeSH terms

Substances

LinkOut - more resources