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. 1985 Feb 15;226(1):217-23.
doi: 10.1042/bj2260217.

The purification of shikimate dehydrogenase from Escherichia coli

S Chaudhuri, J R Coggins

The purification of shikimate dehydrogenase from Escherichia coli

S Chaudhuri et al. Biochem J. .

Abstract

A procedure was developed for the purification of shikimate dehydrogenase from Escherichia coli. Homogeneous enzyme with specific activity 1100 units/mg of protein was obtained in 21% overall yield. The subunit Mr estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate was 32 000. The native Mr, estimated by gel-permeation chromatography on a TSK G2000SW column, was also 32 000. E. coli shikimate dehydrogenase is therefore a monomeric NADP-linked dehydrogenase.

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References

    1. J Bacteriol. 1966 Apr;91(4):1494-508 - PubMed
    1. Nature. 1979 Feb 8;277(5696):486-9 - PubMed
    1. J Bacteriol. 1969 Jul;99(1):222-30 - PubMed
    1. J Bacteriol. 1979 Jun;138(3):933-43 - PubMed
    1. J Biol Chem. 1982 Feb 10;257(3):1491-500 - PubMed

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