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. 1985 Apr;61(2):123-6.
doi: 10.1136/sti.61.2.123.

Typing Haemophilus ducreyi by indirect immunofluorescence assay

L Slootmans, D A Vanden Berghe, P Piot

Typing Haemophilus ducreyi by indirect immunofluorescence assay

L Slootmans et al. Genitourin Med. 1985 Apr.

Abstract

Rabbits were inoculated with untreated whole cells of 16 strains of Haemophilus ducreyi. Homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. No cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. A large diversity in types of antigen was observed among the strains of H ducreyi. Antiserum from two antigens (35000 and 3138), however, reacted with all 16 H ducreyi antigens tested. At least nine of the strains of H ducreyi examined could be identified as determining the type of antigen.

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