Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Apr;48(1):165-8.
doi: 10.1128/iai.48.1.165-168.1985.

Cloning the gene for Congo red binding in Shigella flexneri

P A Daskaleros, S M Payne

Cloning the gene for Congo red binding in Shigella flexneri

P A Daskaleros et al. Infect Immun. 1985 Apr.

Abstract

The ability to bind the dye Congo red from agar medium is associated with virulence of Shigella species. DNA sequences conferring this property have been cloned from a large, 140-kilobase plasmid of Shigella flexneri into a plasmid vector. This recombinant plasmid does not fully restore virulence to S. flexneri isolates which have lost the large plasmid. This indicates that other genes present on the 140-kilobase plasmid must also be required for virulence of S. flexneri. The cloned fragment contains a copy of the insertion sequence IS1 closely linked to the gene for Congo red binding.

PubMed Disclaimer

References

    1. J Mol Biol. 1970 Oct 14;53(1):159-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. J Bacteriol. 1976 Apr;126(1):166-76 - PubMed
    1. Infect Immun. 1977 Oct;18(1):94-8 - PubMed

Publication types

LinkOut - more resources