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. 1985 Jan;403(1):105-8.
doi: 10.1007/BF00583289.

Microprocessor controlled trituration device for the dissociation of cardiac and other tissues

N B Datyner, G A Gintant, I S Cohen

Microprocessor controlled trituration device for the dissociation of cardiac and other tissues

N B Datyner et al. Pflugers Arch. 1985 Jan.

Abstract

The construction of an inexpensive microprocessor controlled piston/cylinder used in the dissociation of canine cardiac Purkinje fibres is described. The microprocessor, interfaced to a linear actuator, controls the motion of a piston using the higher level language "Tiny BASIC" and two machine language routines. Pressure changes generated by the piston are transferred to the lumen of a pipette. Movement of enzymatically-treated tissues through the pipette orifice facilitates dissociation into single cells. With this device, specific agitation protocols for different quantities and types of tissues are possible.

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