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[Preprint]. 2024 Jun 1:2024.05.27.596060.
doi: 10.1101/2024.05.27.596060.

Oxygen Sensor-Guided Fine Needle Biopsy Studies of Human Cancer Xenografts in Mice

Robert C McDonald  1 , Andrew H Fischer  2 , Mary Rusckowski  3
Affiliations

Oxygen Sensor-Guided Fine Needle Biopsy Studies of Human Cancer Xenografts in Mice

Robert C McDonald et al. bioRxiv. .

Abstract

An oxygen sensor-mounted fine-needle biopsy tool was used for in vivo measurement of oxygen levels in tumor xenografts. The system provides a means of measuring the oxygen content in harvested tumor tissue from specific locations. Oxygen in human tumor xenografts in a murine model was observed for over 1 min. Tissues were mapped in relation to oxygen tension (pO2) readings and sampled for conventional cytological examination. Careful modeling of the pO2 readings over 60 seconds yielded a diffusion coefficient for oxygen at the sensor tip, providing additional diagnostic information about the tissue before sampling. Oxygen level measurement may provide a useful adjunct to the use of biomarkers in tumor diagnosis.

Keywords: adenocarcinoma; breast carcinoma; hypoxia; oxygen diffusion; tumor xenograft in mice.

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Figures

Figure 1.
Figure 1.
Bench setup with animal positioned for OGBN measurements
Figure 2.
Figure 2.
Human colon cancer xenograft, obtained using an oxygen sensor-coupled Fine Needle Aspirated (FNA) needle used for these studies, displayed in a non-embedded, Papanicolaou-stained ThinPrep slide. There is an intimate admixture of viable cells with euchromatic nuclei (some shown with long-thin arrows) and dead cells (a few shown with bold arrowheads) as indicated by their smaller darker pycnotic nuclei.
Figure 3.
Figure 3.
Percent O2 saturation in 4-week-old MDA-MB-231 xenograft; white areas represent needle-torn tissue
Figure 4.
Figure 4.
Tissue oxygen measured over 1 minute prior to tissue extraction from left flank LS-174T xenograft
Figure 5.
Figure 5.
Tissue oxygen measured over 1 minute prior to tissue extraction from right flank LS-174T xenograft
Figure 6.
Figure 6.
Xenograft colon tumor samples obtained by an oxygen sensor-coupled FNA (fine needle aspiration) needle from a left flank as shown in ThinPrep slides, Papanicolaou-stained at 400X magnification. On the left is a mostly necrotic sample (Test 2), with only small pycnotic nuclei or no residual nuclei. On the right is a mostly viable tumor sample (Test 4).
Figure 7.
Figure 7.
Showing complex, double exponential relationship between pO2 and the rate at which sensor reaches steady state
See this image and copyright information in PMC

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