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. 2024 Jun 10;19(6):e0303419.
doi: 10.1371/journal.pone.0303419. eCollection 2024.

Uncovering the genetic diversity and adaptability of Butuo Black Sheep through whole-genome re-sequencing

Zengwen Huang  1 , Jing Wang  1 , Dongming Qi  1 , Xiaoyan Li  1 , Jinkang Wang  2 , Jingwen Zhou  3 , Yan Ruan  2 , Youse Laer  2 , Zhangjia Baqian  3 , Chaoyun Yang  1
Affiliations

Uncovering the genetic diversity and adaptability of Butuo Black Sheep through whole-genome re-sequencing

Zengwen Huang et al. PLoS One. .

Abstract

The Butuo Black Sheep (BBS) is well-known for its ability to thrive at high altitudes, resist diseases, and produce premium-quality meat. Nonetheless, there is insufficient data regarding its genetic diversity and population-specific Single nucleotide polymorphisms (SNPs). This paper centers on the genetic diversity of (BBS). The investigation conducted a whole-genome resequencing of 33 BBS individuals to recognize distinct SNPs exclusive to BBS. The inquiry utilized bioinformatic analysis to identify and explain SNPs and pinpoint crucial mutation sites. The findings reveal that reproductive-related genes (GHR, FSHR, PGR, BMPR1B, FST, ESR1), lipid-related genes (PPARGC1A, STAT6, DGAT1, ACACA, LPL), and protein-related genes (CSN2, LALBA, CSN1S1, CSN1S2) were identified as hub genes. Functional enrichment analysis showed that genes associated with reproduction, immunity, inflammation, hypoxia, PI3K-Akt, and AMPK signaling pathways were present. This research suggests that the unique ability of BBS to adapt to low oxygen levels in the plateau environment may be owing to mutations in a variety of genes. This study provides valuable insights into the genetic makeup of BBS and its potential implications for breeding and conservation efforts. The genes and SPNs identified in this study could serve as molecular markers for BBS.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Statistic of mutation information.
Fig 2
Fig 2. Enrichment analysis of genes related to UTR mutation.
Fig 3
Fig 3. Enrichment analysis of genes associated with InterIntro mutation.
Fig 4
Fig 4. Enrichment analysis of genes related to exonic mutations.
Fig 5
Fig 5. PPI analysis of hub genes.
See this image and copyright information in PMC

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