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. 1985 Jan;144(1):199-203.
doi: 10.1016/0003-2697(85)90105-8.

In situ detection of beta-lactamase activity in sodium dodecyl sulfate-polyacrylamide gels

P C Tai, N Zyk, N Citri

In situ detection of beta-lactamase activity in sodium dodecyl sulfate-polyacrylamide gels

P C Tai et al. Anal Biochem. 1985 Jan.

Abstract

After beta-lactamase had been denatured by boiling in the presence of sodium dodecyl sulfate (SDS) and then electrophoresed in SDS-polyacrylamide gels, activity could be restored and could be detected in situ as specific molecular species. Renaturation was simple and facilitated by the presence of a carrier protein. The assay was sensitive, detecting 0.8 ng beta-lactamase activity in the gel.

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