Comparative Study
doi: 10.1007/BF01117438.
Stable maintenance in chemostat-grown Escherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene
- PMID: 3886036
- DOI: 10.1007/BF01117438
Item in Clipboard
Comparative Study
Stable maintenance in chemostat-grown Escherichia coli of pBR322 and pACYC184 by disruption of the tetracycline resistance gene
Biosci Rep.
1985 Jan.
Abstract
Plasmid stability was studied in antibiotic-free chemostat cultures. Disruption, either by deletion or insertion, of the tetracycline resistance gene in the EcoR1/EcoRV region of the cloning vector pBR322 or in the HindIII/BamH1 region of pACYC184 yields plasmids markedly more stable than the parent plasmids. Thus, at least for these two instances, cloning of a partitioning (par) locus is not prerequisite for plasmid maintenance.
Similar articles
-
Molecular cloning in plasmid pBR322 giving altered expression of the tetracycline resistance gene.J Gen Microbiol. 1981 Mar;123(1):187-91. doi: 10.1099/00221287-123-1-187. J Gen Microbiol. 1981. PMID: 6275002
-
Expression of tetracycline resistance in pBR322 derivatives reduces the reproductive fitness of plasmid-containing Escherichia coli.Gene. 1985;39(2-3):173-80. doi: 10.1016/0378-1119(85)90311-7. Gene. 1985. PMID: 3005111
-
Simian virus 40 promoters direct expression of the tetracycline gene in plasmid pACYC184.J Virol. 1983 Jan;45(1):478-81. doi: 10.1128/JVI.45.1.478-481.1983. J Virol. 1983. PMID: 6296456 Free PMC article.
-
Organization of transcriptional signals in plasmids pBR322 and pACYC184.Proc Natl Acad Sci U S A. 1981 Jan;78(1):167-71. doi: 10.1073/pnas.78.1.167. Proc Natl Acad Sci U S A. 1981. PMID: 7017708 Free PMC article.
-
Construction and characterization of E. coli promoter-probe plasmid vectors. III. pBR322 derivatives with deletions in the tetracycline resistance promoter region.Gene. 1982 Dec;20(2):291-304. doi: 10.1016/0378-1119(82)90047-6. Gene. 1982. PMID: 6299894
Cited by
-
pBR322 vectors having tetracycline-dependent replication.Plasmid. 2016 Mar-May;84-85:20-6. doi: 10.1016/j.plasmid.2016.02.004. Epub 2016 Feb 11. Plasmid. 2016. PMID: 26876942 Free PMC article.
-
Outer membrane protein PhoE as a carrier for the exposure of foreign antigenic determinants at the bacterial cell surface.Antonie Van Leeuwenhoek. 1991 May;59(4):249-62. doi: 10.1007/BF00583678. Antonie Van Leeuwenhoek. 1991. PMID: 1715682 Review.
-
Perinatal risk factors for pediatric onset type 1 diabetes, autoimmune thyroiditis, juvenile idiopathic arthritis, and inflammatory bowel diseases.Eur J Pediatr. 2021 Jul;180(7):2115-2123. doi: 10.1007/s00431-021-03987-3. Epub 2021 Feb 23. Eur J Pediatr. 2021. PMID: 33624160 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
