An experimental model of chronic renal failure in mice

Abstract

Chronic renal failure (CRF) was induced in CBA/J mice by a combination of surgery and diathermy to the right kidney followed 2 weeks later by complete removal of the left kidney. This procedure resulted in a progressive rise in blood urea nitrogen (BUN) levels accompanied by a fall in packed cell volume (PCV). In a typical group of mice BUN rose to 156.9 +/- 9.1 mg/100 ml and PCV fell to 36.4 +/- 1.9%, 8 weeks after removal of the left kidney, compared to 23.3 +/- 2.8 mg/100 ml and 51.6 +/- 2.1%, respectively, in normal mice. These changes were accompanied by other serum abnormalities in potassium, sodium, calcium, phosphate, chloride, and silicon levels. The surviving renal tissue showed histological changes, and skeletal changes were also evident on radiological examination. Mice in CRF had an increased mean survival time of tail skin allografts and a significant reduction in thymus weight, supporting the use of this model to investigate the well-documented phenomenon of immunosuppression in human patients with uremia.