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. 2024 Jun 12;40(8):239.
doi: 10.1007/s11274-024-04050-7.

Efficient methane production from agro-industrial residues using anaerobic fungal-rich consortia

Nitiya Thongbunrod  1 , Pawinee Chaiprasert  2
Affiliations

Efficient methane production from agro-industrial residues using anaerobic fungal-rich consortia

Nitiya Thongbunrod et al. World J Microbiol Biotechnol. .

Abstract

Anaerobic digestion (AD) emerges as a pivotal technique in climate change mitigation, transforming organic materials into biogas, a renewable energy form. This process significantly impacts energy production and waste management, influencing greenhouse gas emissions. Traditional research has largely focused on anaerobic bacteria and methanogens for methane production. However, the potential of anaerobic lignocellulolytic fungi for degrading lignocellulosic biomass remains less explored. In this study, buffalo rumen inocula were enriched and acclimatized to improve lignocellulolytic hydrolysis activity. Two consortia were established: the anaerobic fungi consortium (AFC), selectively enriched for fungi, and the anaerobic lignocellulolytic microbial consortium (ALMC). The consortia were utilized to create five distinct microbial cocktails-AF0, AF20, AF50, AF80, and AF100. These cocktails were formulated based on varying of AFC and ALMC by weights (w/w). Methane production from each cocktail of lignocellulosic biomasses (cassava pulp and oil palm residues) was evaluated. The highest methane yields of CP, EFB, and MFB were obtained at 337, 215, and 54 mL/g VS, respectively. Cocktails containing a mix of anaerobic fungi, hydrolytic bacteria (Sphingobacterium sp.), syntrophic bacteria (Sphaerochaeta sp.), and hydrogenotrophic methanogens produced 2.1-2.6 times higher methane in cassava pulp and 1.1-1.2 times in oil palm empty fruit bunch compared to AF0. All cocktails effectively produced methane from oil palm empty fruit bunch due to its lipid content. However, methane production ceased after 3 days when oil palm mesocarp fiber was used, due to long-chain fatty acid accumulation. Anaerobic fungi consortia showed effective lignocellulosic and starchy biomass degradation without inhibition due to organic acid accumulation. These findings underscore the potential of tailored microbial cocktails for enhancing methane production from diverse lignocellulosic substrates.

Keywords: Anaerobic fungi; Bioenergy; Biomass sources; Lignocellulose degradation; Methane generation.

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References

    1. Abt B, Han C, Scheuner C, Lu M, Lapidus A, Nolan M, Lucas S, Hammon N, Deshpande S, Cheng JF, Tapia R, Goodwin LA, Pitluck S, Liolios K, Pagani I, Ivanova N, Mavromatis K, Mikhailova N, Huntemann M, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Brambilla EM, Rohde M, Spring S, Gronow S, Goker M, Woyke T, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP, Detter JC (2012) Complete genome sequence of the termite hindgut bacterium Spirochaeta coccoides type strain (SPN1(T)), reclassification in the genus Sphaerochaeta as Sphaerochaeta coccoides comb. nov. and emendations of the family Spirochaetaceae and the genus Sphaerochaeta. Stand Genomic Sci 6(2):194–209. https://doi.org/10.4056/sigs.2796069 - DOI - PubMed - PMC
    1. Adejumo IO, Adebiyi OA (2020) Agricultural solid wastes: causes, effects, and effective management. In: Hosam MS (ed) Strategies of sustainable solid Waste Management. IntechOpen, Rijeka, pp 1–20. https://doi.org/10.5772/intechopen.93601 - DOI
    1. Ahmad A, Ghufran R, Wahid ZA (2011) Bioenergy from anaerobic degradation of lipids in palm oil mill effluent. Reviews Environ Sci Bio/Technology 10(4):353–376. https://doi.org/10.1007/s11157-011-9253-8 - DOI
    1. Akoh CC, Lee GC, Liaw YC, Huang TH, Shaw JF (2004) GDSL family of serine esterases/lipases. Prog Lipid Res 43(6):534–552. https://doi.org/10.1016/j.plipres.2004.09.002 - DOI - PubMed
    1. Alves MM, Pereira MA, Sousa DZ, Cavaleiro AJ, Picavet M, Smidt H, Stams AJ (2009) Waste lipids to energy: how to optimize methane production from long-chain fatty acids (LCFA). Microb Biotechnol 2(5):538–550. https://doi.org/10.1111/j.1751-7915.2009.00100.x - DOI - PubMed - PMC

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