doi: 10.1111/j.1432-1033.1985.tb08838.x.
In vitro transcription initiation from three different Escherichia coli promoters. Effect of supercoiling
- PMID: 3886381
- DOI: 10.1111/j.1432-1033.1985.tb08838.x
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In vitro transcription initiation from three different Escherichia coli promoters. Effect of supercoiling
Eur J Biochem.
.
Free article
Abstract
Transcription initiation from beta-lactamase, tetracycline resistance and RNA 1 promoters, present in plasmid pAT153, were studied employing the abortive initiation technique. Assays appear to be promoter-specific with supercoiled and linear templates. Supercoiling enhances the isomerization rate constant of the open RNA-polymerase--promoter complex formation. Results agree with the in vivo behaviour of the corresponding promoters, and allow us to propose a hypothesis about the effect of supercoiling on transcription initiation.
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