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. 2024;19(8):667-679.
doi: 10.2217/fmb-2023-0271. Epub 2024 Jun 12.

Biosurfactant complexed with arginine has antibiofilm activity against methicillin-resistant Staphylococcus aureus

Fatima Daiana Dias Barroso  1   2 , Lisandra Juvêncio da Silva  1   2 , Helaine Almeida Queiroz  1   2 , Lívia Gurgel do Amaral Valente Sá  1   2   3 , Anderson Ramos da Silva  4 , Cecília Rocha da Silva  1   2 , João Batista de Andrade Neto  1   2   3 , Bruno Coêlho Cavalcanti  1   5 , Manoel Odorico de Moraes  1   5 , Aurora Pinazo  4 , Lourdes Pérez  4 , Hélio Vitoriano Nobre Júnior  1   2
Affiliations

Biosurfactant complexed with arginine has antibiofilm activity against methicillin-resistant Staphylococcus aureus

Fatima Daiana Dias Barroso et al. Future Microbiol. 2024.

Abstract

Aim: The present study investigated the antimicrobial effectiveness of a rhamnolipid complexed with arginine (RLMIX_Arg) against planktonic cells and biofilms of methicillin-resistant Staphylococcus aureus (MRSA). Methodology: Susceptibility testing was performed using the Clinical & Laboratory Standards Institute protocol: M07-A10, checkerboard test, biofilm in plates and catheters and flow cytometry were used. Result: RLMIX_Arg has bactericidal and synergistic activity with oxacillin. RLMIX_Arg inhibits the formation of MRSA biofilms on plates at sub-inhibitory concentrations and has antibiofilm action against MRSA in peripheral venous catheters. Catheters impregnated with RLMIX_Arg reduce the formation of MRSA biofilms. Conclusion: RLMIX_Arg exhibits potential for application in preventing infections related to methicillin-resistant S. aureus biofilms.

Keywords: MRSA; Staphylococcus aureus; biofilms; medical catheter; surfactants.

Plain language summary

[Box: see text].

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Conflict of interest statement

The authors have no competing interests or relevant affiliations with any organization or entity with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties.

Figures

Figure 1.
Figure 1.
Effect of RLMIX_Arg on the cell viability of preformed 48 h biofilms of methicillin-resistant S. aureus (MRSA) in 96-well plates after 24 h of incubation. (A) * p < 0.05 compared with control / ** p < 0.01 compared with control/ *** p < 0.001 compared with control/ # compared with 16 μg/Ml. (B) Chemical structure of the new biosurfactants produced from the conjugation between mono and di-ramnolipids complex with the amino acid arginine, resulting in the mixture RLMIX_Arg. 1: di-ramnolipids complex; 2: mono-ramnolipids complex.
Figure 2.
Figure 2.
Antibiofilm effect of RLMIX_Arg on the cell viability of methicillin-resistant S. aureus (MRSA) in 96-well plates after 48 h of incubation. * p < 0.05 compared with control / ** p < 0.01 compared with control/ *** p < 0.001 compared with control.
Figure 3.
Figure 3.
Effect of RLMIX_Arg on preformed biofilms against methicillin-resistant S. aureus in Jelco 18G intravenous peripheral catheters after 24 h of treatment and colony-forming units (CFU) at different concentrations. (A) * p < 0.05 compared with control / ** p < 0.01 compared with control/ *** p < 0.001 compared with control/ # compared with 16 μg/ml. (B) (b.1) 2000×, scale 50 μm; (b.4) 10,000×, scale 10 μm) untreated catheter. (b.2) 2000×, scale 50 μm; (b.5) 10,000×, scale 10 μm) catheter treated with 8 μg/ml. (b.3) 2000×, scale 50 μm; (b.6) 10,000×, scale 10 μm) catheter treated with 80 μg/ml.
Figure 4.
Figure 4.
Effect of RLMIX_Arg impregnated catheter against methicillin-resistant S. aureus (MRSA). (A) Colony-forming units at different concentrations. (B) Antibiogram test using strain MRSA 2. CFU: Colony-forming units.
Figure 5.
Figure 5.
Effect of RLMIX_Arg against methicillin-resistant S. aureus after 24 h of treatment with RLMIX_Arg, % of propidium iodide positive cells.
Figure 6.
Figure 6.
Effect of RLMIX_Arg against methicillin-resistant S. aureus after 24 h of treatment with RLMIX_Arg, % of TUNEL-positive cells.
See this image and copyright information in PMC

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