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. 2024 Nov;196(11):8366-8380.
doi: 10.1007/s12010-024-04974-8. Epub 2024 Jun 12.

Biogenic Mn Oxide Generation and Mn(II) Removal by a Manganese Oxidizing Bacterium Bacillus sp. Strain M2

Fada Feng  1   2 , Bang Lan  1   2 , Wenming He  1   2 , Xinrong Wen  1   2 , Shanshuai Chen  3 , Changqing Tu  4   5
Affiliations

Biogenic Mn Oxide Generation and Mn(II) Removal by a Manganese Oxidizing Bacterium Bacillus sp. Strain M2

Fada Feng et al. Appl Biochem Biotechnol. 2024 Nov.

Abstract

Mn(II)-oxidizing bacteria (MOB) are widely distributed in natural environments and can convert soluble Mn(II) into insoluble Mn(III) and Mn(IV). The biogenic manganese oxides (BioMnOx) produced by MOB have been considered for remediating heavy metal pollution and degrading organic pollutants in an eco-friendly manner. In this study, a manganese-oxidizing bacterium was isolated from Mn-polluted rivulet sediment and identified as Bacillus sp. strain M2 by PCR, phylogenetic tree construction, transmission electron microscopy (TEM), and physiological and biochemical indices. Strain M2 grew well under Mn(II) stress. BioMnOx with nanosized irregular geometric shapes and loose structures generated by strain M2 were found on the surface of the bacterial cells. The content of Mn in the bacteria was as high as 5.36%. Approximately 71.24% and 47.52% of Mn(II) was oxidized to Mn(III/IV) in the cell and in the deposits, respectively, within 3 d of cultivation with Mn(II). Extracellular enzymes contributed to the Mn removal and oxidation. In conclusion, Bacillus sp. strain M2 has a high potential for use in the remediation of Mn-contaminated sites.

Keywords: Bacillus sp.; Biogenic Mn oxides; Bioremediation; Manganese-oxidizing bacterium; Mn(II) oxidation.

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Conflict of interest statement

Declarations. Ethical Approval: This article does not contain any studies with human participants or animals performed by any of the authors. Consent to Participate: Not applicable. Consent to Publish: All the authors are aware of this submission and agree to its publication. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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