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. 2024 Jun 12;19(6):e0301342.
doi: 10.1371/journal.pone.0301342. eCollection 2024.

Pyramiding of bacterial blight resistance genes into promising restorer BRRI31R line through marker-assisted backcross breeding and evaluation of agro-morphological and physiochemical characteristics of developed resistant restorer lines

Anowara Akter  1 , Lutful Hassan  2 , Sheikh Arafat Islam Nihad  3 , Md Jamil Hasan  1 , Arif Hasan Khan Robin  2 , Mahmuda Khatun  4 , Anika Tabassum  5 , Mohammad Abdul Latif  1
Affiliations

Pyramiding of bacterial blight resistance genes into promising restorer BRRI31R line through marker-assisted backcross breeding and evaluation of agro-morphological and physiochemical characteristics of developed resistant restorer lines

Anowara Akter et al. PLoS One. .

Abstract

BRRI31R is one of the Bangladesh's most promising restorer lines due to its abundant pollen producing capacity, strong restoring ability, good combining ability, high outcrossing rate and genetically diverse from cytoplasmic male sterile (CMS) line. But the drawback of this line is that it is highly susceptible to bacterial blight (BB) disease of rice caused by Xanthomonas oryzae pv. oryzae. The present study highlighted the pyramiding of effective BB resistance genes (xa5, xa13 and Xa21) into the background of BRRI31R, through marker-assisted backcrossing (MABC). Backcross progenies were confirmed and advanced based on the foreground selection of target genes. Pyramided lines were used for pathogenicity test against five Bangladeshi Xanthomonas oryzae (BXo) races (BXo93, BXo220, BXo822, BXo826, BXo887) and confirmed the dominant fertility restore genes, Rf3 and Rf4 and further validated against SNP markers for more confirmation of target resistance genes. All pyramided restorer lines consisted of Xa4 (in built), xa5, xa13, Xa21, and Chalk5 with two fertility restorer genes, Rf3, Rf4. and these restorer lines showed intermediate amylose content (<25%). Restorer lines BRRI31R-MASP3 and BRRI31R-MASP4 showed high levels of resistance against five virulent BXo races and SNP genotyping revealed that these lines also contained a blast resistance gene Pita races. Gene pyramided restorer lines, BRRI31R-MASP3 and BRRI31R-MASP4 can directly be used as a male parent for the development of new BB resistant hybrid rice variety or could be used as a replacement of restorer line of BRRI hybrid dhan5 and 7 to enhance the quality of hybrid seeds as well as rice production in Bangladesh.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Schematic depiction of the introgression of xa5, xa13 and Xa21 into the restorer line.
Fig 2
Fig 2
(a) Gel photographs showing banding pattern of the markers for the four bacterial blight resistance genes (Xa4, xa5, xa13 and Xa21) in the parents (b) Phenotypic screening of selected parents along with resistant and susceptible check against BXo races of bacterial blight pathogen.
Fig 3
Fig 3
Gel image of Xa4 (a), xa5 (b), xa13 (c), Xa21 (d), Rf3 (e) and Rf4 (f) genes in fixed restorer lines of BC3F5 generation. Here, L1:100bp, L2:1kb, L3:50bp, P1:BRRI31R (recipient parent), P2:IRBB60 (donor parent), P3:IRBB4 (resistant check for Xa4 gene), P4:IR24 (susceptible check), P5:IRBB5 (resistant check for xa5 gene), P6:IRBB13 (resistant check for xa13 gene), P7:IRBB21 (resistant check for Xa21 gene), P8:IR96479-81-7-1-1-B-1-1-1-R (RA, restorer allele), P9:IR58025A (NR, non-restorer allele).
Fig 4
Fig 4. Disease reaction of selected pyramided restorer lines along with respective recurrent parent, donor parent and susceptible check against BXo races of bacterial blight pathogen.
Fig 5
Fig 5. Phenotype of the selected five pyramided restorer lines of BC3F5 generation compared with recurrent parent BRRI31R.
See this image and copyright information in PMC

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