Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jun 11;19(1):20220877.
doi: 10.1515/biol-2022-0877. eCollection 2024.

Investigation into the association of FNDC1 and ADAMTS12 gene expression with plumage coloration in Muscovy ducks

Guo-Bo Sun  1 , Yan-Feng Lu  1 , Xiu-Jun Duan  2
Affiliations

Investigation into the association of FNDC1 and ADAMTS12 gene expression with plumage coloration in Muscovy ducks

Guo-Bo Sun et al. Open Life Sci. .

Abstract

To elucidate the molecular genetic mechanisms underpinning feather color in Muscovy ducks. A cohort of 100 Muscovy ducks was meticulously selected for this research. Follicular tissues from ducks exhibiting black and white plumage served as the experimental samples. From these tissues, RNA and proteins were extracted for further analysis. The RNA underwent reverse transcription polymerase chain reaction amplification, followed by validation through western blot assays. The data revealed a significant upregulation in the expression of FN domain-containing protein 1 (FNDC1) and ADAMTS12 genes in Muscovy ducks with white plumage traits as opposed to those with black plumage traits. Specifically, individuals with pure white plumage demonstrated a markedly elevated expression of the FNDC1 gene in comparison to their pure black counterparts. Conversely, expression levels of the ADAMTS12 gene were found to be reduced in ducks with pure black plumage relative to those with pure white plumage. Notably, the expression patterns of FNDC1 and ADAMTS12 genes exhibited inconsistencies between mRNA and protein levels. This study offers significant insights into the molecular genetic mechanisms underlying feather color variation in Muscovy ducks. FNDC1 and ADAMTS12 could be considered potential targets for genetic manipulation or selective breeding strategies aimed at achieving specific feather color phenotypes in Muscovy ducks.

Keywords: ADAMTS12; FNDC1; Muscovy duck; RT-PCR; feather color; protein expression.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: Authors state no conflict of interest.

Figures

None
Graphical abstract
Figure 1
Figure 1
Results of agarose gel electrophoresis. Note: gel plots: Maker: 3 μL, sample: 150 ng; M: Maker; B: black; W: White.
Figure 2
Figure 2
mRNA expression level of FNDC1 and ADAMTS12. **Compared with black. P < 0.01.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Lin J, Guan L, Ge L, Liu G, Bai Y, Liu X. Nanopore-based full-length transcriptome sequencing of Muscovy duck (Cairina moschata) ovary. Poult Sci. 2021;100(8):101246. - PMC - PubMed
    1. Bao X, Song Y, Li T, Zhang S, Huang L, Zhang S, et al. Comparative transcriptome profiling of ovary tissue between black muscovy duck and white muscovy duck with high- and low-egg production. Genes (Basel). 2020;12(1):57. - PMC - PubMed
    1. Sun Y, Wu Q, Lin R, Chen H, Zhang M, Jiang B, et al. Genome-wide association study for the primary feather color trait in a native Chinese duck. Front Genet. 2023;14:1065033. - PMC - PubMed
    1. Guo Q, Jiang Y, Wang Z, Bi Y, Chen G, Bai H, et al. Genome-wide analysis identifies candidate genes encoding feather color in ducks. Genes (Basel). 2022;13(7):1249. - PMC - PubMed
    1. Li S, Wang C, Yu W, Zhao S, Gong Y. Identification of genes related to white and black plumage formation by RNA-Seq from white and black feather bulbs in ducks. PLoS One. 2012;7(5):e36592. - PMC - PubMed

LinkOut - more resources