TLR3, TLR7, and TLR8 genes expression datasets in COVID-19 patients: Influences of the disease severity and gender

Abstract

The prognosis of COVID-19 could influence by innate immune sensors such as toll-like receptors (TLRs). The purpose of this data was to investigate TLR3, 7, and 8 expression levels in COVID-19 patients and their relationship to outcome of disease. 75 confirm COVID-19 were included sequentially and separated into three groups: mild, severe, and critical. Peripheral blood mononuclear cells were isolated from the whole blood, and RNA was then extracted. The qRT-PCR technique was used to examine the expression of TLR3, TLR7, and TLR8 genes. The patients average ages were 52.69 ± 1.9 and 13 of the 25 individuals in each group were male. TLR3 (p < 0.001), TLR7 (p < 0.001), and TLR8 (p < 0.001) expression levels were considerably greater in COVID-19 patients compared to the control group. The findings also showed that individuals with critical and severe COVID-19 disease had significantly greater TLR7 and TLR8 gene expression levels than patients in mild stage of disease (p < 0.05). The data showed a significant difference (p = 0.01) in the TLR3 transcript levels between critical and mild COVID-19 patients. Furthermore, male severe (p = 0.02) and critical (p = 0.008) patients had significantly higher TLR8 expression levels than female patients in terms of gender. TLR3 (p = 0.2) and TLR7 (p = 0.08) transcripts were more elevated in males than females, but not significantly.

Keywords: Coronavirus disease 2019; Severe acute respiratory syndrome coronavirus 2; TLR7; TLR8; Toll-like receptors 3 (TLR3).

Fig. 1

TLR3 (A), TLR7 (B), and TLR8 (C) gene expression in peripheral blood samples from COVID-19 patients and the control group. The expression of TLR3 (A), TLR7 (B), and TLR8 (C) genes was compared between PBMCs from COVID-19 patients and the control group. Fold changes are displayed to show differences in gene expression across groups. Statistically significant variations (p < 0.05) were observed.

Fig. 2

TLR3 A), TLR7 B), and TLR8 C) gene expression was compared in COVID-19 patients during different stages. Fold changes are used to display data. Significant differences across groups (p < 0.05).

Fig. 3

Male and female patients with various illness outcomes were compared in terms of the TLR3 (A), TLR7 (B), and TLR8 (C) genes expression. ns: not significant.

Fig. 4

The mRNA expression levels of TLR3, TLR7, and TLR8 were analysed in male and female COVID-19 patients based on disease outcome and gender. Fig. 4A shows TLR3 expression in male patients. Fig. 4B shows TLR3 in females. Fig. 4C depicts TLR7 levels in males while Fig. 4D shows TLR7 in females. TLR8 expression in males is presented in Fig. 4E and in females in Fig. 4F. Data is presented as fold change compared to control. ``ns'' denotes non-significant differences while ``p < 0.05'' indicates significant differences between specified groups.