doi: 10.1007/BF00583607.
Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope
- PMID: 3887323
- DOI: 10.1007/BF00583607
Item in Clipboard
Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope
Pflugers Arch.
1985 Mar.
Abstract
The design of a small, inexpensive temperature controlled bath (0.25 ml volume) for electrophysiological studies of isolated cells is described. The design creates a uniform temperature (+/- 1 degree C) from ambient temperature to approximately 37 degrees C over a bath chamber area of 0.6 X 1 cm for flow rates from zero to 2 ml/min. Access for microelectrodes and unobstructed preparation viewing with an inverted microscope is afforded by a finely stranded heater sandwiched between two glass cover slips and located beneath the bath chamber. Battery operation of the feedback temperature controller and solution pre-heater minimizes electrical interference.
Similar articles
-
A novel experimental chamber for single-cell voltage-clamp and patch-clamp applications with low electrical noise and excellent temperature and flow control.Pflugers Arch. 1986 May;406(5):536-9. doi: 10.1007/BF00583378. Pflugers Arch. 1986. PMID: 3714452
-
Temperature-controlled perfusion apparatus for microscope using transparent conducting film heater.Am J Physiol. 1989 Jan;256(1 Pt 1):C214-7. doi: 10.1152/ajpcell.1989.256.1.C214. Am J Physiol. 1989. PMID: 2912134
-
A modular perfused chamber for low- and normal-temperature microscopy of living cells.Biotechniques. 2011 Apr;50(4):251-4. doi: 10.2144/000113653. Biotechniques. 2011. PMID: 21548909
-
A perfusion chamber for high-resolution light microscopy of cultured cells.J Microsc. 1988 Jul;151(Pt 1):71-5. doi: 10.1111/j.1365-2818.1988.tb04614.x. J Microsc. 1988. PMID: 3057215
-
A regulated environmental perfusion system for the study of anoxic or hypoxic cultured neurons using microfluorescence imaging and electrophysiology.Pflugers Arch. 1998 May;435(6):775-80. doi: 10.1007/s004240050583. Pflugers Arch. 1998. PMID: 9518505
Cited by
-
Stimulation of cardiac alpha receptors increases Na/K pump current and decreases gK via a pertussis toxin-sensitive pathway.Biophys J. 1988 Aug;54(2):219-25. doi: 10.1016/S0006-3495(88)82950-3. Biophys J. 1988. PMID: 2850027 Free PMC article.
-
Pacemaker current i(f) in adult canine cardiac ventricular myocytes.J Physiol. 1995 Jun 1;485 ( Pt 2)(Pt 2):469-83. doi: 10.1113/jphysiol.1995.sp020743. J Physiol. 1995. PMID: 7545232 Free PMC article.
-
A superfusion bath for single-cell recording with high-precision optical depth control, temperature regulation, and rapid solution switching.Pflugers Arch. 1990 Apr;416(1-2):94-7. doi: 10.1007/BF00370228. Pflugers Arch. 1990. PMID: 2352844
-
A novel experimental chamber for single-cell voltage-clamp and patch-clamp applications with low electrical noise and excellent temperature and flow control.Pflugers Arch. 1986 May;406(5):536-9. doi: 10.1007/BF00583378. Pflugers Arch. 1986. PMID: 3714452
-
Background K+ current in isolated canine cardiac Purkinje myocytes.Biophys J. 1987 Oct;52(4):519-25. doi: 10.1016/S0006-3495(87)83241-1. Biophys J. 1987. PMID: 2445390 Free PMC article.