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. 1985 Mar;403(3):318-23.
doi: 10.1007/BF00583607.

Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope

N B Datyner, G A Gintant, I S Cohen

Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope

N B Datyner et al. Pflugers Arch. 1985 Mar.

Abstract

The design of a small, inexpensive temperature controlled bath (0.25 ml volume) for electrophysiological studies of isolated cells is described. The design creates a uniform temperature (+/- 1 degree C) from ambient temperature to approximately 37 degrees C over a bath chamber area of 0.6 X 1 cm for flow rates from zero to 2 ml/min. Access for microelectrodes and unobstructed preparation viewing with an inverted microscope is afforded by a finely stranded heater sandwiched between two glass cover slips and located beneath the bath chamber. Battery operation of the feedback temperature controller and solution pre-heater minimizes electrical interference.

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References

    1. Am J Physiol. 1977 Nov;233(5):C172-9 - PubMed
    1. Pflugers Arch. 1983 Apr;397(2):158-9 - PubMed

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