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. 2024 Mar 7;12(6):4160-4172.
doi: 10.1002/fsn3.4076. eCollection 2024 Jun.

Curcumin mitigates acrylamide-induced ovarian antioxidant disruption and apoptosis in female Balb/c mice: A comprehensive study on gene and protein expressions

Sanaz Alaee  1   2 , Zahra Khodabandeh  2 , Mahintaj Dara  2 , Elham Hosseini  3   4 , Mona Sharma  5
Affiliations

Curcumin mitigates acrylamide-induced ovarian antioxidant disruption and apoptosis in female Balb/c mice: A comprehensive study on gene and protein expressions

Sanaz Alaee et al. Food Sci Nutr. .

Abstract

Curcumin is known for its antioxidant properties. This study aimed to investigate the impact of curcumin on acrylamide (ACR)-induced alterations in the first-line antioxidant defense of ovarian tissue. Female Balb/c mice were divided into control, ACR (50 mg/kg), ACR/CUR100 (received Acr + curcumin100 mg/kg), and ACR/CUR200 (Acr + curcumin 200 mg/kg) groups, and received oral treatments for 35 days. Evaluation of antioxidant enzyme expression (Sod, Cat, Gpx genes), pro-apoptotic gene expressions (Bax, Caspase 3), and anti-apoptotic gene expression (Bcl2l1) at mRNA and protein levels was done. Percentage of apoptotic cells using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed. The model group (ACR) showed decreased mRNA expression of Sod, Cat, and Gpx genes compared with the control group. Treatment with two different doses of curcumin (CUR100 and CUR200) significantly increased Sod, Cat, and Gpx gene expression, with CUR200 demonstrating significant recovery. SOD, CAT, and GPX protein levels were similar to mRNA expression trends, significantly increased with curcumin administration. Acrylamide exposure significantly increased Bax and Caspase 3 expression and decreased Bcl2l1 gene expression leading to a notable rise in apoptosis in ACR group as compared to the control group. Conversely, curcumin administration, significantly reduced Bax and Caspase 3 expressions, with an increase in Bcl2l1expression, though not statistically significant. TUNEL assay revealed a substantial decrease in apoptosis in curcumin-received groups. In our study, ACR exposure adversely affected ovarian antioxidant defense thereby leading to increased pro-apoptotic markers. Notably, curcumin treatment effectively mitigated these effects, restored antioxidant potential, and reduced acrylamide-induced toxicity in female mouse ovaries.

Keywords: acrylamide; antioxidants; curcumin; female infertility.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

FIGURE 1
FIGURE 1
The relative expression of Sod, Cat, and Gpx genes at the mRNA level of ovaries in the curcumin treatment versus the untreated groups versus control. Bar represented by mean ± SD and the p < .05 was considered as significant. a: vs. Control group. b: vs. Acr group. c: vs. Acr/CUR100 group. d: vs. Acr/CUR200 group.
FIGURE 2
FIGURE 2
Representative pictures of immunofluorescence staining for SOD (shiny green) proteins in ovarian sections of mice, counterstained with DAPI for nuclei (blue), and merged pictures, in control, acrylamide (Acr), Acrylamid+curcumin100 (Acr/CUR100), and Acrylamid+curcumin200 (Acr/CUR200) groups.
FIGURE 3
FIGURE 3
Representative pictures of immunofluorescence staining for CAT (shiny green) proteins in ovarian sections of mice, counterstained with DAPI for nuclei (blue), and merged pictures, in control, acrylamide (Acr), Acrylamid+curcumin100 (Acr/CUR100), and Acrylamid+curcumin200 (Acr/CUR200) groups.
FIGURE 4
FIGURE 4
Representative pictures of immunofluorescence staining for GPX (shiny green) proteins in ovarian sections of mice, counterstained with DAPI for nuclei (blue), and merged pictures, in control, acrylamide (Acr), Acrylamid+curcumin100 (Acr/CUR100), and Acrylamid+curcumin200 (Acr/CUR200) groups.
FIGURE 5
FIGURE 5
Quantification of Immunofluorescence staining for SOD, CAT, and GPX protein expression. Data expressed as means ± SD. Data are statistically significant at p < .05. a: vs. Control group. b: vs. Acr group. c: vs. Acr/CUR100 group. d: vs. Acr/CUR200 group.
FIGURE 6
FIGURE 6
The relative expression of Bax, Bcl2l1, and Caspase 3 genes at the mRNA level of ovaries in the curcumin treatment versus the untreated groups versus control. Bar represented by mean ± SD and the p < .05 was considered as significant. a: vs. Control group. b: vs. Acr group. c: vs. Acr/CUR100 group. d: vs. Acr/CUR200 group.
FIGURE 7
FIGURE 7
(a) Representative pictures of immunofluorescence staining for TUNEL (green fluorescence) in ovarian sections of mice, counterstained with DAPI for nuclei (blue), and merged pictures, in control, acrylamide (Acr), Acrylamid+curcumin100 (Acr/CUR100), and Acrylamid+curcumin200 (Acr/CUR200) groups. (b) Quantification of Immunofluorescence staining for SOD, CAT, and GPX protein expression. Data expressed as means ± SD. Data are statistically significant at p < .05. a: vs. Control group. b: vs. Acr group. c: vs. Acr/CUR100 group. d: vs. Acr/CUR200 group.
FIGURE 8
FIGURE 8
(a) Schematic representation of the impact of acrylamide exposure on ovarian tissue. (b) The therapeutic potential of curcumin in restoring antioxidant balance and mitigating toxicity in female mice.
See this image and copyright information in PMC

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