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. 2024 Aug;68(8):254-266.
doi: 10.1111/1348-0421.13155. Epub 2024 Jun 14.

Antibiotic susceptibility and genome analysis of Enterococcus species isolated from inpatients in one hospital with no apparent outbreak of vancomycin-resistant Enterococcus in Japan

Ayumi Fujii  1   2 , Miki Kawada-Matsuo  2   3 , Mi Nguyen-Tra Le  2   3 , Kanako Masuda  2   3 , Kayoko Tadera  4 , Yujin Suzuki  2 , Saki Nishihama  2   5 , Junzo Hisatsune  3   6 , Yo Sugawara  6 , Seiya Kashiyama  4 , Hideki Shiba  5 , Tomonao Aikawa  1 , Hiroki Ohge  3   7 , Motoyuki Sugai  3   6 , Hitoshi Komatsuzawa  2   3
Affiliations

Antibiotic susceptibility and genome analysis of Enterococcus species isolated from inpatients in one hospital with no apparent outbreak of vancomycin-resistant Enterococcus in Japan

Ayumi Fujii et al. Microbiol Immunol. 2024 Aug.

Abstract

To prevent nosocomial infection, it is important to screen for potential vancomycin-resistant Enterococcus (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1 to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing were performed. A total of 164 isolates, including Enterococcus faecium (41 isolates), Enterococcus faecalis (80 isolates), Enterococcus raffinosus (11 isolates), Enterococcus casseliflavus (nine isolates), Enterococcus avium (12 isolates), Enterococcus lactis (eight isolates), Enterococcus gallinarum (two isolates), and Enterococcus malodoratus (one isolate), were analyzed. We found one vanA-positive E. faecium, which was already informed when the patient was transferred to the hospital, nine vanC-positive E. casseliflavus, and two vanC-positive E. gallinarum. E. faecium isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and E. faecalis isolates showed resistance to minocycline (49.4%). Ampicillin- and levofloxacin-resistant E. faecium had multiple mutations in penicillin-binding protein 5 (PBP5) (39/39 isolates) and ParC/GyrA (21/36 isolates), respectively. E. raffinosus showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and E. lactis showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes optrA and cfr(B) were found only in one isolate of E. faecalis and E. raffinosus, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.

Keywords: Enterococcus; PBP5; antibiotic resistance genes; antibiotic susceptibility; hospital surveillance; vancomycin‐resistant Enterococcus.

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References

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