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. 2024 Jun 14;43(7):171.
doi: 10.1007/s00299-024-03254-3.

Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome

Lamiaa M Mahmoud  1 , Manjul Dutt  2   3
Affiliations

Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome

Lamiaa M Mahmoud et al. Plant Cell Rep. .

Abstract

A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

Keywords: Albino phenotype; CRISPR/Cas9; Citrus; Genome editing; Marker-free; Precision breeding.

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