Culture, sex and social context influence brain-to-brain synchrony: an fNIRS hyperscanning study

Abstract

Background: Unique interpersonal synchrony occurs during every social interaction, and is shaped by characteristics of participating individuals in these social contexts. Additionally, depending on context demands, interpersonal synchrony is also altered. The study therefore aims to investigate culture, sex, and social context effects simultaneously in a novel role-play paradigm. Additionally, the effect of personality traits on synchrony was investigated across cultures, and a further exploratory analysis on the effects of these variables on pre- and post-session empathy changes was conducted.

Methods: 83 dyads were recruited in two waves from Singapore and Italy and took part in a within-subjects session where they interacted with each other as themselves (Naturalistic Conversation) and as others (Role-Play and Role Reversal). Big Five Inventory (administered pre-session) and Interpersonal Reactivity Index (administered pre- and post-session) were used as measures of personality and empathy respectively, while synchrony was measured using hyperscanning functional near-infrared spectroscopy in the prefrontal cortex. After data-preprocessing and preliminary analyses, a mixture of multiple linear regression and exploratory forward stepwise regression models were used to address the above study aims.

Results: Results revealed significant main and interaction effects of culture, sex and social context on brain-to-brain synchrony, particularly in the medial left cluster of the prefrontal cortex, and a unique contribution of extraversion and openness to experience to synchrony in the Italian cohort only. Finally, culture-driven differences in empathy changes were identified, where significant increases in empathy across sessions were generally only observed within the Singaporean cohort.

Conclusions: Main findings indicate lowered brain-to-brain synchrony during role-playing activities that is moderated by the dyad's sex make-up and culture, implying differential processing of social interactions that is also influenced by individuals' background factors. Findings align with current literature that role-playing is a cognitively demanding activity requiring greater levels of self-regulation and suppression of self-related cognition as opposed to interpersonal co-regulation characterized by synchrony. However, the current pattern of results would be better supported by future studies investigating multimodal synchronies and corroboration.

Keywords: Big five inventory; Culture; Empathy; Interpersonal reactivity index; Interpersonal synchrony; Personality; Prefrontal cortex; Role-play; Sex; fNIRS.

Fig. 1

fNIRS prefrontal cortex configuration and its corresponding international 10–20 EEG position. .Note: Purple lines indicate approximate areas where channels are formed

Fig. 2

Experimental protocol. the procedure is color coded as follows: orange (online questionnaires) and gray (lab)

Fig. 3

Schematic of anatomical clustering in the prefrontal cortex Note: Clusters are color coded: anterior left (green); anterior right (orange); medial left (yellow); medial right (blue). In this schematic, channels 9 and 12 are not used (gray)

Fig. 4

Two-way interaction between cohort and condition

Fig. 5

Two-way interaction between cohort and sex

Fig. 6

Two-way interaction between sex and condition

Fig. 7

Three-way interaction between cohort, sex and condition. (Left) Data from Singapore cohort. (Right) Data from Italy cohort