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. 2024 Jun 14;18(1):115.
doi: 10.1186/s13065-024-01193-4.

Determination of levofloxacin, norfloxacin, and moxifloxacin in pharmaceutical dosage form or individually using derivative UV spectrophotometry

K H Elgendy  1 , M Zaky  2 , Alaa Eldin Mohamed Mahmoud Altorky  2 , S Fadel  2
Affiliations

Determination of levofloxacin, norfloxacin, and moxifloxacin in pharmaceutical dosage form or individually using derivative UV spectrophotometry

K H Elgendy et al. BMC Chem. .

Abstract

Purpose: In this study, first, second, third, and fourth-order derivative spectrophotometric methods utilizing the peak-zero (P-O) and peak-peak (P-P) techniques of measurement were developed for the determination of levofloxacin, norfloxacin, and moxifloxacin. These methods were applied to their combined pharmaceutical dosage form or individually for levofloxacin, norfloxacin, and moxifloxacin.

Methods: Linearity was established in the concentration range of 2-20 µg/mL. The procedures are simple, quick, and precise. The developed methods are sensitive, accurate, and cost-effective, demonstrating excellent correlation coefficients (R2 = 0.9998) and mean recovery values ranging from 99.20% to 100.08%, indicating a high level of precision.

Results: The developed approach was effectively employed to determine the levofloxacin, norfloxacin, and moxifloxacin content in commercially available pharmaceutical dosages.

Conclusions: Statistical analysis and recovery tests confirmed the method's linearity and accuracy. The results suggest that this method can be utilized for routine analysis in both bulk and commercial formulations. The simplicity, accuracy, and cost-effectiveness of the developed methods make them valuable for pharmaceutical analysis.

Keywords: Derivative Uv spectrophotometry; Levofloxacin; Moxifloxacin; Norfloxacin; Simultaneous estimation; Validation.

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Conflict of interest statement

The authors declare no competing interest.

Figures

Fig. 1
Fig. 1
Absorbance and derivative spectra of a Gaussian band
Fig. 2
Fig. 2
Spectrums of levofloxacin, norfloxacin, and moxifloxacin in zero order
Fig. 3
Fig. 3
First (l) second (2), third (3) and fourth (4) derivative spectra of levofloxacin
Fig. 4
Fig. 4
First (l) second (2), third (3) and fourth (4) derivative spectra of norfloxacin
Fig. 5
Fig. 5
First (l) second (2), third (3) and fourth (4) derivative spectra of moxifloxacin
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